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开发犬丸有机体,用于评估环境暴露的影响

Lei Yin¹, Zoey Hsuan Hsiao¹, Chelin Hu²

¹Reprotox Biotech LLC, United States.

Reproductive toxicology (Elmsford, N.Y.)

|November 29, 2025

概括

研究人员开发了一种3D犬丸器官模型,用于研究男性生殖毒性. 这种以道德为基础的模型支持胚胎细胞的维持,并有助于评估生殖毒素,为动物试验提供了替代方案.

科学领域:

生殖生物学生殖生物学
毒理学毒理学毒理学
3D有机体技术 3D有机体技术

背景情况:

包括精子生成在内的男性生殖系统的体外建模是复杂的.
伦理考虑和3R (替换,减少,改进) 需要替代的测试方法.

研究的目的:

开发和验证一个3D犬丸器官模型.
评估其用于评估男性生殖毒素的有用性和遵守3Rs.

主要方法:

伦理来源的犬丸被用于通过酶消化,微型成型和细胞外矩阵嵌入来创建3D器官.
分析了器官的发育,细胞类型的存在 (细菌,塞尔托利,莱迪格细胞),基因表达和对化 (CdCl2) 的反应.

主要成果:

器官体表现出生长和复杂的形态,保持关键的丸细胞类型和生殖细胞分化标志物.
基因表达分析显示,丸功能标志物的动态调节,包括GATA4上调.
暴露于CdCl2降低了细胞活力,并诱导了DNA损伤 (γ-H2AX表达),证明了毒理反应.

结论:

一个功能性的3D犬丸器官模型成功生成和验证.
该模型支持胚胎细胞的维持,并允许对生殖毒素进行机械评估.
它是用于生殖毒性测试的体内方法的科学稳健和道德替代方案.

关键词:

这是3Rs原则.狗类模型模型新方法方法论 (NAMs)生殖性毒性生殖性毒性精子发生是精子生成.丸的器官类动物.

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