相关实验视频

Updated: May 12, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

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优选TMT:针对有限样本的优化和缩小的TMT标签策略

Michele Martins1, Fábio César Sousa Nogueira1, Magno Junqueira1

  • 1Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil.

Journal of proteomics
|November 29, 2025
PubMed
概括

我们优化了Tandem质量标签 (TMT) 标签,以显著降低试剂成本和质谱蛋白质组学样本需求. 这种具有成本效益的方法保持了高的标签效率,使先进的蛋白质组分析可用于有限的样本研究.

关键词:
标签上的异酸标签.标签效率的提高 标签效率的提高量化蛋白质组学是一种定量化的蛋白质组学.TMTT TMTT 是一个很好的方法.

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