在单原子桥式纳米酶中进行工程直接电子转移,以在中性pH下增强氧化酶类活性
Chaolei Hua1, Mingyang Jiang2, Guan Liu2
1Materials Artificial Intelligence Center, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
Biosensors & bioelectronics
|November 29, 2025
概括
这项研究介绍了一种新的黄金单原子纳米酶在二氧化 (MnO2-Au SAN) 上,可以在中性pH下增强催化活性. 这一进步改善了生物传感应用,如流感A病毒检测.
科学领域:
- 材料科学 材料科学 材料科学
- 纳米技术纳米技术
- 生物化学 生物化学
背景情况:
- 传统的纳米酶在中性pH下表现出有限的催化活性,阻碍了生理应用.
- 在生理条件下开发具有增强活性的纳米酶对于生物医学应用至关重要.
研究的目的:
- 设计和描述一个支持MnO2的金单原子纳米酶 (MnO2-Au SAN),在中性pH下具有增强的氧化酶类活性.
- 研究电子金属支相互作用 (EMSI) 以及其在提高催化效率方面的作用.
- 为了证明MnO2-Au SAN在侧流免疫试验中用于检测流感A病毒的应用.
主要方法:
- 结辅助的光化学还原用于MnO2-Au SAN合成.
- 谱学表征和运动测量以研究催化活性.
- 激素清除和电子自旋共振 (ESR) 分析以阐明催化机制.
- 在侧流免疫试验中用于检测流感A病毒的应用.
主要成果:
- 由于强大的EMSI和高效的电子转移,MnO2-Au SAN在中性pH下显著增强了氧化酶类活性.
- 实现TMB氧化低迈克利斯常数 (0.040mM),超过了许多报告的纳米酶.
- 在中性条件下,证明增强了洞和基的产生.
- 在侧流免疫试验中启用了20 pg/mL的流感A病毒检测极限.
结论:
- 基于EMSI的策略有效地提高了在生理pH下纳米酶活性.
- 原子级电子转移对于模仿自然酶反应至关重要.
- 在生理环境中,MnO2-Au SAN显示出敏感生物传感应用的巨大潜力.
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