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Updated: Jan 9, 2026

Using Multilayered Hydrogel Bioink in Three-Dimensional Bioprinting for Homogeneous Cell Distribution

Using Multilayered Hydrogel Bioink in Three-Dimensional Bioprinting for Homogeneous Cell Distribution

Published on: May 2, 2020

生物相容的墨水优化使得功能体积生物打印能够使用异形印刷.

Erik Brauer¹, Aiste Balciunaite², Matthias R Kollert^3,4

¹Center for the Science of Materials Berlin (CSMB) and Department of Chemistry, Humboldt University, 12489, Berlin, Germany.

Advanced materials (Deerfield Beach, Fla.)

|November 30, 2025

概括

此摘要是机器生成的。

本研究定义了细胞兼容的Xolography生物墨水的设计原则,确保高细胞活力和功能,用于组织工程和再生医学中先进的生物打印应用.

关键词:

通过3D打印打印3D打印.影音摄影 (Xolography) 是一种影音摄影技术.生物混合机器人机器人技术生物打印是一种生物打印技术.组织工程是组织工程.

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Bioprinting of Cartilage and Skin Tissue Analogs Utilizing a Novel Passive Mixing Unit Technique for Bioink Precellularization

Published on: January 3, 2018

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科学领域:

生物技术是生物技术.
材料科学材料科学材料科学
再生医学是一种再生医学.

背景情况:

假影技术在制造中提供了高精度和速度,但由于生物相容性差,在生物打印方面面临限制.
了解Xolography生物墨中的细胞活力约束对于其在组织工程中的应用至关重要.

研究的目的:

建立细胞兼容的Xolography生物墨水的基本设计原则.
定义生物墨水配方的框架,使无支制造能够保持细胞活力和功能.

主要方法:

系统地研究细胞外pH值,度和细胞活力和功能上的 lysosomotropic 压力.
使用各种小鼠和人类细胞系,初级细胞和诱导多能干细胞 (iPSC) 衍生细胞,验证生物墨水配方.

主要成果:

定义了生物墨水配方框架,允许快速,无支的制造,细胞活力>90%.
与三甲醇胺不同,BisTris证明了作为共同发起剂的生物相容性,维持细胞代谢活性和分化.
成功制造复杂的细胞载荷结构,包括ECM形成,内皮芽和iPSC衍生的肝细胞维护.

结论:

异形生物打印是一种可行的平台,用于创建复杂的,细胞载荷的结构.
已建立的生物墨设计原则促进了组织工程,器官芯片模型和再生医学中的应用.