相关实验视频

Updated: Jan 9, 2026

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer

Published on: August 2, 2024

功能性动态调节结肠直肠癌发病和转移能力的新型MIR-7974调节轴

Yu-Hao Liu¹, Yi-Tung Chen², Yu-Chang Chen³

¹Department of Cell and Molecular Biology, College of Medicine, Chang Gung University, Taoyuan, Taiwan.

Biomedical journal

|November 30, 2025

概括

微RNA-7974 (miR-7974) 通过抑制细胞循环调节器促进结肠直肠癌 (CRC) 的生长,但通过向MYO1E.减少转移. 动态miR-7974表达影响CRC进展和患者存活率.

科学领域:

分子生物学分子生物学
基因组学就是基因组学.
在瘤学瘤学.

背景情况:

大肠直肠癌 (CRC) 是一种普遍存在的恶性瘤,转移和复发率高,需要新的治疗策略.
奥米克数据分析的进步已经阐明了瘤发生的复杂调节网络,为癌症治疗提供了新的途径.

研究的目的:

为了确定涉及结直肠癌 (CRC) 进展的新型微RNA (miRNAs).
研究miR-7974在CRC生长和转移中的调节网络和功能作用.

主要方法:

对CRC组织和正常样本进行系统的转录组分析,以识别差异表达的miRNAs.
用RT-qPCR,基于细胞的测定和异种移植模型来评估miR-7974在瘤进展中的功能.
RNA测序,记者测定和功能救援实验,以界定miR-7974调节网络,重点关注CDKN1A和MYO1E.

主要成果:

miR-7974在CRC中表现出明显的表达模式,在早期阶段具有高水平,并在后期转移阶段的丰度下降.
miR-7974通过降低CDKN1A的调节来促进CRC的生长,并通过向MYO1E来减少迁移/入侵.
miR-7974抑制了表皮细胞到介质细胞的转变 (EMT),保持了增殖表皮细胞表型,并与有利的患者生存结果相关联.

结论:

miR-7974调节CRC中的EMT可塑性,促进增殖,同时抑制转移潜力.
miR-7974的动态表达及其基因调节提供了对转移性潜在收购的机制性见解.
miR-7974对CRC生长的功能影响及其预后意义突显了它在癌症发展和miRNome动态中的作用.

关键词:

癌症的发展癌症的发展结肠直肠癌是一种癌症.潜在的转移性转移.据报道,米R-797474号机型是一架飞机.

更多相关视频

Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts

Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts

Published on: April 29, 2014

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...

mTOR Signaling and Cancer Progression

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...

Adaptive Mechanisms in Cancer Cells

Adaptive Mechanisms in Cancer Cells

Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...

The Tumor Microenvironment

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...