在标准化和质量控制中使用HPTLC的Sphaeranthus indicus Linn. 使用互补的溶剂系统
Sumedh Sanjay Joshi1, Aniket Jadhav2, Akshay Charegaonkar2
1Department of Dravyaguna, All India Institute of Ayurveda, Gautampuri awas, Sarita Vihar, Mathura road, 110076 New Delhi, India.
Annales pharmaceutiques francaises
|November 30, 2025
概括
高性能薄层染色学 (HPTLC) 指纹的Sphaeranthus indicus是使用补充开发溶剂 (CDS) 系统开发的. 这种可靠的方法确保了药用植物的准确认证和质量控制.
科学领域:
- 植物化学 植物化学
- 分析化学 分析化学
- 药理学是指药理学,即药理学是指药理学.
背景情况:
- 斯法兰修斯指针线. 是一种传统的阿育吠陀药用植物,以抗炎,肝脏保护和神经活性特性而闻名.
- 它的治疗作用与各种植物成分有关,如酸乳,黄类药物和固醇.
- 准确的化学分析对于Sphaeranthus indicus的真实性和质量控制至关重要.
研究的目的:
- 为了建立一个全面的和可重复的高性能薄层染色学 (HPTLC) 指纹为Sphaeranthus indicus.
- 使用补充开发溶剂 (CDS) 系统来分析植物的各种溶剂提取物.
- 为了确保HPTLC指纹采集过程中的可靠性和可重复性,用于Sphaeranthus indicus.
主要方法:
- 制备了五种Sphaeranthus indicus的提取物 (甲醇,n-hexane,乙,石油,50%的乙醇).
- 在60 F254板上使用CAMAG HPTLC PRO系统与三种溶剂系统 (LPDS,MPDS,HPDS) 进行HPTLC分析.
- 视觉化涉及紫外线和白光,有或没有衍生 (甲硫酸,10%甲醇硫酸),然后进行方法验证.
主要成果:
- 在所有溶剂系统中获得了明显的HPTLC指纹,解决了不同的植物化学品类.
- 低极性 (LPDS) 分离了非极性化合物 (类,类固醇),中极性 (MPDS) 分离了中极性化合物 (黄类),高极性 (HPDS) 检测了极性化合物.
- 观察到一致的Rf值 (0.15,0.43),衍生增强斑点可见性和颜色差异化;方法验证证实高可重复性,精度和准确性.
结论:
- 采用补充发育溶剂 (CDS) 系统开发的HPTLC方法提供了Sphaeranthus indicus的强大而详细的指纹档案.
- 这种HPTLC指纹技术是有效的质量控制,标准化和标记物化合物的识别在Sphaeranthus indicus草药配方.
- 该方法支持Sphaeranthus indicus在传统和现代应用中的可靠认证和质量评估.
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