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纳米纤维集成PD-1介导向和基于CRISPR/Cas9的CD47编辑,用于双重免疫检查点阻断

Huimin Kong¹, Siqing Wang², Chenya Zhuo³

¹Laboratory of Biomaterials and Translational Medicine, Department of Ultrasound, Center for Nanomedicine, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China; Department of Ophthalmology and Visual Sciences and Wisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, WI 53706, USA.

Journal of controlled release : official journal of the Controlled Release Society

|November 30, 2025

概括

这项研究介绍了BITE,这是一种纳米平台,针对PD-1/PD-L1和CD47通路来增强癌症免疫疗法. BITE纳米纤维重新编程瘤细胞,使其对免疫系统更加可见,从而导致显著的瘤回归.

科学领域:

生物医学工程生物医学工程
纳米医学是一种纳米医学.
癌症免疫学癌症免疫学

背景情况:

癌症免疫疗法面临着由于瘤逃避机制的挑战.
关键的免疫逃避途径包括PD-1/PD-L1检查点和CD47检查点.
不要吃我,不要吃我
信号. 信号. 这是信号.

研究的目的:

开发和评估一个新的纳米平台,BITE (仿生免疫准和编辑),用于免疫检查点的双重封锁.
评估BITE在增强先天性和适应性抗瘤免疫力的有效性.

主要方法:

工程生物模拟纳米纤维 (BITE) 显示PD-1用于瘤向.
利用BITE提供的CRISPR/Cas9基因编辑来淘汰瘤细胞中的CD47.
通过使用小鼠瘤模型在体外和体内评估BITE的性能.

主要成果:

BITE选择性地向PD-L1阳性瘤和破坏CD47表达.
BITE诱导了巨细胞显著的瘤细胞细胞分解.
通过BITE的双重阻断促进了T细胞和M1巨细胞的透,导致瘤回归和延长小鼠的存活时间.

结论:

BITE纳米平台为先天性和适应性免疫的协同作用提供了概念证明.
这种双重功能的方法克服了免疫抵抗力,对癌症治疗有希望.
BITE代表了纳米医学中针对癌症向治疗的多功能策略.

关键词:

生物仿真纳米微小圈这就是CRISPR/Cas9的作用.免疫检查站封锁免疫检查站封锁非病毒基因编辑.有针对性的交付目标.

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