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快速表型抗菌敏感性测试使用多通道大体积散射成像和贝叶斯高斯过程模型

Jiapei Jiang¹, Shuang Zhou², Michelle McBride¹

¹Biodesign Center for Bioelectronics and Biosensors, Arizona State University, Tempe, Arizona 85287, United States.

Analytical chemistry

|December 1, 2025

概括

快速抗微生物敏感性测试 (AST) 对于治疗多抗药性 (MDR) 感染至关重要. 一个新的大体积散射成像 (LVSim) 系统在2小时内提供精确的表型AST和最小抑制度 (MIC) 结果.

科学领域:

微生物学微生物学
生物医学工程生物医学工程
传染性疾病传染性疾病

背景情况:

耐多药性 (MDR) 细菌感染对全球健康构成重大威胁,特别是在性休克等关键条件下.
对MDR感染的有效抗生素治疗方法的发现延迟与患者死亡率的增加直接相关.
目前的抗微生物敏感性测试 (AST) 方法可能耗时,妨碍及时的临床决策.

研究的目的:

开发和验证一种新的多通道大体积散射成像 (LVSim) 系统,用于快速和精确的表型AST.
允许同时监测多个样本/药物条件,以进行高效的测试.
为了快速确定指导抗生素治疗的最小抑制度 (MIC).

主要方法:

开发一种多通道大容量散射成像 (LVSim) 系统,能够同时监测多达八种条件.
应用贝叶斯的高斯过程模型来分析细菌的长期生长动态.
使用*Escherichia coli*和*Pseudomonas aeruginosa* (包括一种缓慢生长的MDR临床分离物) 进行验证.

主要成果:

对于测试的细菌菌株,LVSim系统成功地在2小时内实现了快速MIC确定.
该系统在表型AST中表现出精度,区分了敏感性概况.
同时监测能力允许对多种抗生素治疗进行有效评估.

结论:

多重化LVSim系统代表了快速表型AST的重大进步.
这项技术为对抗具有挑战性的多药耐药性细菌感染提供了有希望的解决方案.
使用LVSim更快地确定MIC可以导致更及时,更有效的患者治疗,可能降低死亡率.

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