使用胺酸修饰的电支架恢复非矿化牙的功能恢复:多式体外表征的多式体外表征
Aruna Krishnan1, Sandhya Raghu1, Govindaraj Perumal2
1Department of Conservative Dentistry and Endodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India.
Journal of oral biology and craniofacial research
|December 1, 2025
概括
用胺酸修饰的纳米纤维支架通过促进有组织的矿物沉积和恢复机械性能,显著改善了牙的重矿化. 这种仿生方法为修复性牙科应用提供了一个有希望的替代方案.
科学领域:
- 生物材料科学 生物材料科学
- 恢复性牙科 恢复性牙科
- 纳米技术 纳米技术
背景情况:
- 由于其复杂的组成,登丁的重矿化具有挑战性.
- 电纳米纤维支架为矿物沉积提供了一个仿生平台.
- 结合像谷氨酸这样的生物分子可以增强脚手架的重矿化潜力.
研究的目的:
- 通过使用含有谷氨酸酸的聚烯酸/纳米酸酸 (PCL/nHA/Glu) 支架来评估酸去矿化牙的重矿化.
- 为了评估脚手架应用后牙的表面和机械恢复.
- 描述功能化的脚手架所促进的矿物沉积过程.
主要方法:
- 制造PCL/nHA和PCL/nHA/Glu纳米纤维支架. 制造PCL/nHA和PCL/nHA/Glu纳米纤维支架.
- 将脚手架应用于非矿物化的人类牙盘,并在模拟体液中浸泡7,14和28天.
- 使用SEM,EDS,AFM和纳米印记进行分析,以评估形态,组成,地形和机械性能.
主要成果:
- SEM和EDS证实了PCL/nHA/Glu组的增强和有组织的矿物沉积,具有显著的管管封闭.
- 在功能化的脚手架组中,AFM表明随着时间的推移,表面粗度降低.
- 纳米印证明了弹性模量和硬度的改善,表明14日后机械性能恢复.
结论:
- 谷氨酸功能化显著增强牙重矿化和机械性质恢复.
- 氨基酸修饰的支架作为牙再生的生物活性平台.
- 这些支架显示出在修复牙科中作为传统材料的替代品的潜力.
相关概念视频
01:31Essential Minerals for Bone Health
The minerals contained in all of the food we consume are essential for our organ systems. However, certain essential minerals, such as calcium, phosphorus, magnesium, manganese, and fluoride, largely affect bone health.
Calcium and Phosphorus
Calcium is a critical component of bones, especially in the form of calcium phosphate and calcium carbonate. Since the body cannot make calcium, it must be obtained from the diet. However, calcium cannot be absorbed from the small intestine without...
Calcium and Phosphorus
Calcium is a critical component of bones, especially in the form of calcium phosphate and calcium carbonate. Since the body cannot make calcium, it must be obtained from the diet. However, calcium cannot be absorbed from the small intestine without...
01:22EDTA: Chemistry and Properties
Polydentate ligands are most widely used in complexometric titrations because they form more stable complexes with the metal ions than mono- or bidentate ligands due to the chelate effect. Examples of polydentate ligands are ethylenediaminetetraacetic acid (EDTA), crown ethers, and cryptands. The most important feature of optimal polydentate ligands is the ability to form 1:1 complexes in a single-step process. Amino carboxylic acid derivatives are frequently used as complexing agents. EDTA is...
01:26EDTA: Auxiliary Complexing Reagents
EDTA titrations are usually carried out in highly basic conditions, where the fully deprotonated form of EDTA, Y4−, actively complexes with the free metal ions in the solution. Several metal ions precipitate as hydrous oxide (hydroxides, oxides, or oxyhydroxides) under these conditions, lowering the concentration of free metal ions in the solution. For this reason, auxiliary complexing agents or ligands such as ammonia, tartrate, citrate, or triethanolamine are used in EDTA titrations to...
01:18Effects of EDTA on End-Point Detection Methods
Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a result, EDTA...
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a result, EDTA...
01:19Masking and Demasking Agents
EDTA titrations may necessitate masking and demasking agents to temporarily protect a particular metal ion in a mixture from the EDTA reaction. These agents facilitate the sequential analysis of the metal ions by forming stable complexes with some—but not all—metal ions during certain steps.
There are many masking agents, such as cyanide, fluoride, triethanolamine, thiourea, and 2,3-bis(sulfanyl)propan-1-ol (formerly 2,3-dimercapto-1-propanol), with the masking agent chosen based on the metal...
There are many masking agents, such as cyanide, fluoride, triethanolamine, thiourea, and 2,3-bis(sulfanyl)propan-1-ol (formerly 2,3-dimercapto-1-propanol), with the masking agent chosen based on the metal...
01:24Types of Reversible Electrodes
For electrode reversibility to be maintained, all the reactants and products involved in the half-reaction must be present at the electrode. There are several types of reversible electrodes (half-cells).In metal-metal-ion electrodes, a metal balances electrochemically with a solution of its own ions. Examples are Cu2+|Cu and Zn2+|Zn. Metals that react with the solvent, like group 1 and most group 2 metals, which react with water, and zinc, which reacts with aqueous acidic solutions, cannot be...