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结构建模和生物化学表征MSMEG_0748,一个来自Mycobacterium smegmatis的MoxR ATPase

Ju Eun Lee¹, So Yeon Lee¹, Hyo Guk Kim¹

¹Department of Biological Sciences and Biotechnology, Chungbuk National University, Cheongju 28644, Republic of Korea.

Computational and structural biotechnology journal

|December 1, 2025

概括

MSMEG_0748,是Mycobacterium smegmatis中的一个MoxR型ATPase,充当一个陪伴者. 它形成六合体,解ATP,并与MSMEG_0751相互作用,形成金属酶成熟所必需的系统.

科学领域:

微生物学微生物学
结构生物学结构生物学
生物化学生物化学

背景情况:

结核菌菌通过适应而在宿主条件下生存.
一氧化碳脱酶 (CO-DH) 基因集群通过代谢CO和NO来帮助生存.
假设MSMEG_0748,一个MoxR型AAA+ ATPase,是 CO-DH 成熟的陪伴者.

研究的目的:

在Mycobacterium smegmatis中从结构和功能上描述MSMEG_0748的特征.
研究MSMEG_0748在金属酶成熟中的作用.

主要方法:

预测AlphaFold3结构和序列对齐.
使用多角度光散射的尺寸排除色谱 (SEC-MALS).
生物化学测定 (ATP水解) 和生物物理分析.
同免疫沉实验.

主要成果:

在溶液中,MSMEG_0748形成一个稳定的六合体.
结构模型揭示了保留的沃克A/B图案和MoxR特定的H2I.
证实了ATP水解活性,并且依赖于催化谷氨酸E146.
MSMEG_0748与MSMEG_0751直接发生相互作用.

结论:

MSMEG_0748具有固有的ATP水解活性.
复杂的MSMEG_0748-MSMEG_0751功能作为一个MoxR-VWA陪伴系统.
这一系统对于mycobacteria中的金属酶成熟至关重要.

关键词:

这是一种ATPase检测法.阿尔法结构预测预测在CO-DH成熟期.莫克斯R ATP 酶的作用这种细菌是Mycobacterium smegmatis.

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