相关实验视频

Updated: Jan 9, 2026

Isolation of Mouse Interstitial Valve Cells to Study the Calcification of the Aortic Valve In Vitro

Isolation of Mouse Interstitial Valve Cells to Study the Calcification of the Aortic Valve In Vitro

Published on: May 10, 2021

综合的多组学分析揭示了动脉狭窄症中关键的枢纽基因和机制

Feng-Xia Wang¹, Shuai Liu¹, Hao-Qiang Guo²

¹First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.

Frontiers in cardiovascular medicine

|December 1, 2025

概括

在大动脉狭窄 (AS) 中识别致病基因至关重要. 这项研究发现了包括MMP9和PLAU在内的16个枢纽基因,有助于诊断AS并揭示它们在炎症中的作用.

科学领域:

心血管生物学心血管生物学
分子病理学分子病理学
基因组学就是基因组学.

背景情况:

大动脉狭窄 (AS) 是结构性心脏病的重要危险因素,需要确定其潜在的遗传因素.
了解AS的致病基因可以为心脏病理和治疗策略提供新的见解.

研究的目的:

确定参与大动脉狭窄病变的关键基因.
探索已识别的基因的诊断潜力.
通过多组学方法阐明AS的分子机制.

主要方法:

使用 edgeR.R. 的差异基因表达分析 (批量-RNAseq).
基因本体学 (GO),KEGG途径和基因组丰富分析 (GSEA).
蛋白质基因测序,通过五种算法识别枢纽基因,以及ROC曲线分析.
单细胞RNA测序 (scRNAseq) 详细介绍AS的病变发生.

主要成果:

转录组分析显示MMP9,CXCL8和SPP1的高表达,AS中活性缺氧,炎症和纤维化.
蛋白质组学显示在化AS中增强了缺氧,TNF-α信号传递和细胞外矩阵形成.
包括ITGB3,ITGAV和MMP9在内的16个枢纽基因显示出高诊断效率 (AUC>0.75). 证实MMP9和PLAU在化门中表达高.
scRNAseq显示MMP9和PLAU在内皮细胞,单细胞和巨细胞中,与单细胞/巨细胞分化,脂质代谢和炎症有关.

结论:

已识别的16个枢纽基因显示出有助于主动脉狭窄诊断的前途.
MMP9和PLAU可以通过调节单细胞和巨细胞的炎症前驱活动,促进AS的发展.

关键词:

大动脉狭窄症 (大动脉狭窄症)石灰化是指化.蛋白质组是一种蛋白质组.单细胞转录组学单细胞转录组学转录组 (transcriptome) 是一个转录组.

更多相关视频

Isolation of Human Primary Valve Cells for In vitro Disease Modeling

Isolation of Human Primary Valve Cells for In vitro Disease Modeling

Published on: April 16, 2021

Isolation and Characterization of Primary Rat Valve Interstitial Cells: A New Model to Study Aortic Valve Calcification

Isolation and Characterization of Primary Rat Valve Interstitial Cells: A New Model to Study Aortic Valve Calcification

Published on: November 20, 2017

相关概念视频

Imaging Studies for Cardiovascular System VI: Calcium -Scoring CT

Imaging Studies for Cardiovascular System VI: Calcium -Scoring CT

Calcium-Scoring CT ScanA calcium-scoring CT scan, also known as coronary artery calcium (CAC) scan, detects calcium deposits in the coronary arteries. This test assesses the risk of coronary artery disease (CAD), which can lead to cardiovascular events such as angina, heart failure, and sudden cardiac arrest.A calcium-scoring CT scan is generally recommended for individuals at intermediate risk of CAD without symptoms. It includes:Men aged 40-75 and women aged 50-75: Especially those with a...

Coronary Artery Disease II: Pathophysiology

Coronary Artery Disease II: Pathophysiology

Coronary Artery Disease (CAD) originates from a series of events that impair the function of coronary arteries, the blood vessels responsible for delivering oxygen-rich blood to the heart muscle. The pathophysiology of CAD is closely linked to atherosclerosis, a chronic inflammatory and lipid-driven condition affecting the vascular endothelium.1. Endothelial DamageThe process begins with damage to the vascular endothelium, which serves as a protective barrier between the blood and the vessel...

Aortic Regurgitation I: Introduction

Aortic Regurgitation I: Introduction

IntroductionAortic regurgitation is characterized by the backward flow of blood from the aorta into the left ventricle during diastole and arises from the improper closure of the aortic valve. This condition results in left ventricular volume overload and can stem from both acute and chronic etiologies, each contributing uniquely to the disease's progression and symptomatology.Acute and Chronic CausesAcute aortic regurgitation often results from events that suddenly impair the integrity of the...

Mechanism of Angiogenesis

Mechanism of Angiogenesis

Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...