基C-甘氨基转移酶 能够在有机溶剂中进行C-甘氨基化
Onur Kırtel1, Lea Helena Strother1, Natalia Putkaradze1
1The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Søltofts Plads 220, Lyngby DK-2800, Denmark.
ACS omega
|December 1, 2025
概括
研究人员从植物中发现了新型耐盐C-糖转移酶,这些植物有效地产生稳定的C-糖. 这些酶在有机溶剂中表现出增强的活性,改善了工业糖化工艺的产量.
科学领域:
- 生物催化和酶工程 生物催化和酶工程
- 植物生物化学 植物生物化学
- 合成生物学 合成生物学
背景情况:
- C-糖转移酶 (家族1) 通过将糖与芳香环结合,合成稳定的C-糖.
- 受体基质的低水溶性限制了生物催化甘化产量.
- 耐盐生物通常具有在有机溶剂中具有增强稳定性的酶.
研究的目的:
- 为了发现和描述新型的C-glycosyltransferases从状细胞.
- 评估酶在有机溶剂中的效率和稳定性,以改善糖化.
- 在工业应用中探索性C-糖转移酶的潜力.
主要方法:
- 测序化物基因组的挖掘,以确定C-glycosyltransferase候选物.
- 使用纯化的酶,生物催化转化花素到C-葡萄糖.
- 酶的表征包括最佳温度,pH值和溶剂耐受性.
- 在不同的基质和酶度下评估酶性能.
主要成果:
- 鉴定和描述了三种新型的性C-葡萄糖转移酶.
- 酶可以有效地将佛洛转化为具有高区域选择性的C-葡萄糖体.
- 在有乙或甲醇的情况下,转换产量显著增加 (高达1563%) .
- 在55-65°C和pH值8.7-10.0.0的温度下观察到最佳活性.
- 一种酶 (PaCGT) 在低酶和高基质条件下表现出显著的稳定性.
结论:
- 基C-糖转移酶是强大的生物催化剂,在有机溶剂中具有增强的活性.
- 这些酶提供了一个有希望的解决方案,以克服基质可溶性限制在糖化.
- 它们的独特特性使得它们在工业糖化处理过程中非常有价值,因为工业糖化需要高度的基质.
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