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评估用于制备酶固定生物反应器的不同基质的性能

Mahbuba Jannat¹, Swarnila Chakma¹, Md Junayed¹

¹Department of Chemical Engineering, Bangladesh University of Engineering and Technology, Dhaka-1000, Bangladesh.

|December 1, 2025

概括

与管相比,玻璃幻灯片在生物反应器中提供优越的稳定性和可重复使用性,用于固定过氧化酶 (HRP) 酶,这使得它们非常适合降解应用.

科学领域:

生物技术是生物技术.
酶固定化酶固定化
生物反应器设计设计

背景情况:

酶固定化需要稳定和可重复使用的基质,以实现高效的酶生物反应器.
卜过氧化酶 (HRP) 是各种生物催化应用的关键酶,包括污染物降解.

研究的目的:

评估玻璃幻灯片和管作为HRP固定的基板.
为了比较HRP固定生物反应器在不同基板上的稳定性,可重复使用性和存储性.

主要方法:

基质处理 (3-氨基) -三基 (APTES),其次是德克斯聚甲 (DPA) 或甲 (GA).
在经过处理的玻璃片和管上固定HRP.
醇降解测试以确定酶活性,加载,可重复使用性和储存稳定性.

主要成果:

在玻璃片上固定的HRP降解了21%的,而管降解了10%.
用DPA处理的玻璃幻灯片显示HRP最大负载 (6%) 和活动 (2.52 U).
玻璃滑板生物反应器在8个周期和4个月的储存后保持了100%的活性,而管在8个周期后保持了91.5%的活性,并在4个月后 (GA处理) 保持了90%.

结论:

玻璃幻灯片,特别是DPA处理的玻璃幻灯片,由于稳定性和可重复使用性提高,是HRP固定的优质基板.
用DPA终结的玻璃片为HRP固定生物反应器提供了出色的准备使用基板潜力.
基质的选择显著影响生物反应器应用中的酶固定的性能和寿命.

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