一种替代的UV-Vis光谱法,用于精确量化丰富微生物生物质中的总量
Caroline Schmitz1, Daniel Kuhn1, Guilherme Schwingel Henn1,2
1Food Biotechnology Laboratory, University of Vale do Taquari - Univates, Lajeado, RS ZC 95914-014, Brazil.
ACS omega
|December 1, 2025
概括
一种新的紫外线可见光谱法准确量化微生物生物质中的总 (Se). 这种具有成本效益的方法非常适合食品和生物技术行业的质量控制.
科学领域:
- 分析化学 分析化学
- 生物技术是生物技术.
背景情况:
- 精确量化总 (Se) 对于评估其在丰富微生物生物质中的生物利用性和有效性至关重要.
- 像ICP-MS这样的现有方法是准确的,但昂贵,并且无法用于许多研究和工业环境.
研究的目的:
- 验证一种简单,快速,经济高效的紫外线可见光谱光度法,用于在丰富微生物生物质中总量定量.
- 通过将其与ICP-MS和经过认证的参考材料进行比较来评估该方法的可靠性和稳定性.
主要方法:
- 微波辅助的酸性消化,使有机完全矿化.
- 使用-与粉反应的紫外线可见光谱相.
- 使用化*Lactiplantibacillus plantarum*和*Saccharomyces* spp.进行线性,检测/量化极限,精度和准确性的验证.
主要成果:
- 该方法表现出良好的线性,精度和准确性,复原率在细菌中为97-111%,在酵母中为99-101%.
- 超声波预处理导致因干扰粉-复合物的过高估计.
- 在没有超声波预处理的情况下,在20°C达到最佳效果.
结论:
- 经过验证的紫外线可见光谱法为微生物生物质提供了准确的总量定量.
- 该协议是ICP-MS的成本效益和可访问的替代方案,用于食品和生物技术行业的质量控制和研究应用.
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