Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Volatilization01:10

Volatilization

5.0K
Volatilization gravimetry is an analytical technique that measures the mass lost due to the volatilization of the substance. This technique is used to estimate the amount of volatile material in a sample. To perform this method, heat a known amount of the sample to a high temperature in a crucible or other suitable vessel. The volatile substance in the sample evaporates, and the vapor is completely expelled from the crucible either by heating the sample or bubbling a stream of inert gas through...
5.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Integrative single-cell and bulk transcriptomics reveal cuproptosis subtypes, prognostic models, and COL5A1 as a key gene in glioblastoma.

Cellular signalling·2026
Same author

Gut microbiota and metabolic disruption induced by food chain-transferred nanoplastics: A mediating role of CYP26A1 in retinoic acid toxicity.

Ecotoxicology and environmental safety·2026
Same author

Associations of adverse childhood experiences and obesity with multimorbidity in middle-aged and older adults in China.

Child abuse & neglect·2025
Same author

Increased N-glycosylation of PSMA by GnT-V enhances tumor malignancy through interacting with JAK2 and the subsequent STAT3-mediated transcriptional activation in prostate cancer.

International journal of biological macromolecules·2025
Same author

Depressive symptoms changes in the new-onset stroke patients: A cross-lagged panel network analysis.

Journal of affective disorders·2025
Same author

From insects to mammals! Tissue accumulation and transgenerational transfer of micro/nano-plastics through the food chain.

Journal of hazardous materials·2024
Same journal

Mechanical forces of beating heart suppress cancer growth.

Mechanobiology in medicine·2026
Same journal

Mechanobiology of hypertrophic chondrocyte mineralization: Biomechanical regulation and therapeutic implications in skeletal disorders.

Mechanobiology in medicine·2026
Same journal

Mechanochemical bistability as a principle of robust morphogenesis.

Mechanobiology in medicine·2026
Same journal

Substrate pre-stretch reprograms macrophage behavior through surface topographical remodeling.

Mechanobiology in medicine·2026
Same journal

Mechanosensitive reactive oxygen Species:A precision medicine target for oxidative stress-related diseases.

Mechanobiology in medicine·2026
Same journal

Viscous drag-induced self-regeneration of laser-dissected actin stress fibers: A potential mechanism for mechanical memory.

Mechanobiology in medicine·2026
查看所有相关文章

相关实验视频

Updated: Apr 30, 2026

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

5.3K

为Visium空间转录学处理矿化组织的实用协议.

Jixing Miao1, Mingjie Ma1, Yuhan Guo2

  • 1Department of Orthopedics, The First Hospital of China Medical University, Shenyang, Liaoning, 110001, China.

Mechanobiology in medicine
|December 1, 2025
PubMed
概括
此摘要是机器生成的。

这项研究为骨组织提供了一个优化的空间转录组学协议. 它使用莫尔斯溶液进行快速脱和专门的幻灯片来保持RNA质量并改善矿物化样本中的基因检测.

关键词:
骨头 骨头 骨头 骨头脱过程中的脱.降解RNA的降解RNA的降解空间转录组学 空间转录组学这就是Visium Visium.

更多相关视频

Author Spotlight: Exploring Advanced Therapeutic Targets in Osteosarcoma Through Spatial Transcriptomics
07:43

Author Spotlight: Exploring Advanced Therapeutic Targets in Osteosarcoma Through Spatial Transcriptomics

Published on: May 3, 2024

4.2K
En face Cryosectioning of Mouse Retina for High-dimensional Spatial Molecular Analysis
08:57

En face Cryosectioning of Mouse Retina for High-dimensional Spatial Molecular Analysis

Published on: July 8, 2025

1.1K

相关实验视频

Last Updated: Apr 30, 2026

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

5.3K
Author Spotlight: Exploring Advanced Therapeutic Targets in Osteosarcoma Through Spatial Transcriptomics
07:43

Author Spotlight: Exploring Advanced Therapeutic Targets in Osteosarcoma Through Spatial Transcriptomics

Published on: May 3, 2024

4.2K
En face Cryosectioning of Mouse Retina for High-dimensional Spatial Molecular Analysis
08:57

En face Cryosectioning of Mouse Retina for High-dimensional Spatial Molecular Analysis

Published on: July 8, 2025

1.1K

科学领域:

  • 生物技术是生物技术.
  • 分子生物学分子生物学
  • 基因组学就是基因组学.

背景情况:

  • 矿物化组织的空间转录组因脱过程中RNA降解而具有挑战性.
  • 传统的EDTA脱协议是漫长的,并损害RNA完整性.
  • 需要改进的方法来进行高分辨率的基因表达分析.

研究的目的:

  • 使用10x Genomics Visium开发和验证一个优化的协议,用于矿物化组织的空间转录学.
  • 为了提高RNA保存和转录捕获效率在骨和骨折样本.
  • 克服分析骨组织基因表达的预分析性局限性.

主要方法:

  • 用莫尔斯溶液取代EDTA脱,以快速处理 (<24小时).
  • 使用了SCHOTT NEXTERION® Slide H (3-D水凝涂层) 来增强脆弱骨段的粘附性.
  • 将该协议应用于完整和骨折的小鼠大腿骨,用于空间转录组学分析.

主要成果:

  • 莫尔斯的溶液使得快速脱,同时保持RNA质量 (有利的DV200值).
  • 优化的协议显著提高了Visium V2和Visium HD平台的转录捕获效率.
  • 视觉V2的平均值为5639个基因/点;视觉HD的平均值为170个基因/8μm bin.

结论:

  • 开发的协议有效地克服了矿化组织空间转录学的主要预分析障碍.
  • 这种方法可以实现骨的高分辨率空间转录基因分析,并提高数据质量.
  • 优化的工作流程可以更深入地了解骨组织的分子格局.