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Piper chaba通过银纳米颗粒的介导合成来控制多抗药性腹致便病原体

Masuma Anzuman^1,2, Md Abdullah Al Mashud^3,4, Rawnak Jahan Rakhy Moni^1,2

¹Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia, 7003, Bangladesh.

Biochemistry and biophysics reports

|December 1, 2025

概括

来自Piper chaba叶的绿色合成的银纳米颗粒有效控制了诸如大肠杆菌 (E. coli) 这样的多抗药性便病原体. 这些纳米颗粒还表现出抗氧化和低细胞毒性,为传统抗生素提供了有希望的替代品.

科学领域:

生物医学科学生物医学科学
纳米技术纳米技术
微生物学微生物学

背景情况:

纳米颗粒的植物介导生物合成是生物医学研究的一个不断增长的领域.
多抗药性 (MDR) 腹致便病原体对全球健康构成重大威胁.
需要新的治疗策略来打击抗生素耐药性.

研究的目的:

为了隔离和识别MDR便病原体.
使用Piper chaba叶提取物合成银纳米粒子 (AgNPs).
评估合成的AgNP的抗菌,抗氧化和细胞毒性质.

主要方法:

使用生物化学试验和16S rRNA基因测序,分离和识别便病原体.
使用Piper chaba叶提取物合成AgNP的绿色合成.
使用UV-Vis光谱,FTIR,TEM,DLS和XRD进行AgNP的表征.
通过最小抑制度 (MIC) 评估的抗菌活性.
使用DPPH试验评估的抗氧化活性.
细胞毒性使用盐水死亡率测定 (BSLA) 进行评估.

主要成果:

孤立的MDR病原体:球菌 (Enterococcus gallinarum),肺炎菌 (Klebsiella pneumoniae) 和大肠杆菌 (Escherichia coli) 这三种病原体.
有特征的球形AgNP (平均尺寸为16.06nm),SPR峰值在440nm.
AgNP表现出显著的抗氧化活性 (69.28% RSA) 和低细胞毒性 (LC50 = 912.01 μg/mL).
有效抑制MDR病原体:K. pneumoniae和大肠杆菌的MIC为4μg/mL,E. gallinarum的MIC为16μg/mL.

结论:

Piper chaba 叶片介导的AgNP对MDR便病原体表现出强大的抗菌活性.
合成的AgNP具有有利的抗氧化剂和低细胞毒性特征.
绿色合成的AgNP代表了对抗抗生素耐药性感染的有希望的替代治疗剂.

关键词:

抗生素耐药性抗生素耐药性细胞毒性细胞毒性便病原体的病原体绿色合成是一种绿色合成.银纳米颗粒银纳米粒子

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