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Updated: Jan 9, 2026

PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase
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单个突变到氨酸或谷氨酸提高了灵活的双域蛋白质的结晶性和晶体衍射性质

Christina Geerds1, Hartmut H Niemann1

  • 1Department of Chemistry, Bielefeld University, Universitätsstrasse 25, 33615 Bielefeld, Germany.

Acta crystallographica. Section F, Structural biology communications
|December 1, 2025
PubMed
概括

单一的表面突变显著增强了蛋白质结晶和衍射. 这些针对Listeria monocytogenes入侵蛋白InlB392的定向变化克服了灵活性问题,产生了高分辨率的蛋白质晶体结构.

关键词:
晶体接触器的使用情况多域蛋白质是多个领域的蛋白质.突变点突变点突变点突变点突变蛋白质结晶化 蛋白质结晶化表面突变是表面突变.减少了表面的减少.

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