病毒lncRNAs的系统识别和表征表明宿主lncRNAs的广泛结构模仿
Ping Fu1,2, Zena Cai1,3, Ruina You1
1Bioinformatics Center, College of Biology, Hunan Provincial Key Laboratory of Medical Virology, Hunan Research Center of the Basic Discipline for Cell Signaling, Hunan University, No. 27 Tianma Road, Yuelu District, Changsha, 410082, China.
Briefings in bioinformatics
|December 1, 2025
概括
这项研究确定了来自25种物种的5,053种新型病毒长非编码RNA (vlncRNAs),揭示了新的结构和功能. 许多vlncRNA模仿人类的lncRNA,可能通过miRNA海绵调节生物过程.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 生物信息学是一种生物信息学.
背景情况:
- 病毒长非编码RNA (vlncRNAs) 在病毒感染中至关重要,但人们对其了解甚少.
- 有限的识别和表征阻碍了对其角色的研究.
研究的目的:
- 在各种病毒物种中识别和表征新型vlncRNA.
- 探索 vlncRNA 和人类 lncRNA 之间的结构和功能相似之处.
- 为 vlncRNAs建立一个全面的数据库.
主要方法:
- 第三代测序用于vlncRNA识别.
- RT-qPCR用于验证选定的 vlncRNAs.
- 生物信息分析用于结构和序列比较,miRNA结合和拼接模式.
主要成果:
- 鉴定了来自25种病毒物种的5,053种新型vlncRNA,主要来自dsDNA病毒.
- 发现~1%的vlncRNAs有注释的RNA家族,表明了新的结构.
- 从15种人类病毒中发现的772个vlncRNA结构上模仿人类lncRNAs (hlncRNAs),并可能充当miRNA海绵.
- 在vlncRNA拼接中观察到替代的第一个外因子的流行.
结论:
- 这项研究显著扩大了已知的vlncRNAs谱系,提供了对其多样性和起源的见解.
- 由vlncRNAs对hlncRNAs的结构模仿表明保存的调节机制和在宿主-病原体相互作用中的潜在作用.
- 开发的vlncRNA数据库作为未来关于vlncRNAs和病毒病原学的研究的宝贵资源.
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