在死细胞灭绝过程中,活细胞对溶酶体膜通透性的成像
Katia S G Andrade1, John Mably2, Da-Zhi Wang2
1Center for Regenerative Medicine, Heart Institute, Department of Internal Medicine, Morsani College of Medicine, University of South Florida; kgomesandrade@usf.edu.
Journal of visualized experiments : JoVE
|December 1, 2025
概括
溶解体膜通透性 (LMP) 是死细胞灭绝的关键,导致细胞死亡. 活细胞成像有效地追踪LMP和溶酶体功能障碍在这个调节的亡过程中.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 亡是一种受调节的亡形式,涉及细胞膜破裂.
- lysosomal膜通透性 (LMP) 是一个早期的,关键的事件,在细胞亡中发生细胞破裂.
- LMP释放 lysosomal 酶到细胞质中,驱动细胞死亡.
研究的目的:
- 为了研究 lysosomal 膜通透 (LMP) 在亡中的作用.
- 为了证明活细胞成像对于在死过程中实时检测LMP的实用性.
- 为了描述 lysosomal 功能障碍在死的时间和程度.
主要方法:
- 利用活细胞成像技术实时监测亡.
- 使用pH敏感的LysoTracker染料来评估溶酶体pH的变化.
- 使用光素标记的德克斯珠来检测溶酶体载荷泄漏到细胞质中.
主要成果:
- 观察到LysoTracker光度的逐渐减少,表明了溶酶体功能障碍.
- 检测到光的扩散到细胞质中,证实LMP和货物释放.
- 建立了观察到的光变化和死的进展之间的相关性.
结论:
- 活细胞成像是一种强大的工具,用于研究 lysosomal 参与死亡的死亡.
- 该方法允许精确跟踪 lysosomal 功能障碍的动态在死亡过程中.
- 这些发现有助于理解亡机制,并确定潜在的治疗点.
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