相关实验视频

Updated: Jan 9, 2026

Virus-induced Gene Silencing VIGS in Nicotiana benthamiana and Tomato

Virus-induced Gene Silencing VIGS in Nicotiana benthamiana and Tomato

Published on: June 10, 2009

双子病毒载体:从基因沉默到合成生物学

Yi Zhang¹, Shulin Deng²

¹Guangdong Provincial Key Laboratory of Applied Botany & Key Laboratory of National Forestry and Grassland Administration on Plant Conservation and Utilization in Southern China, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.

Biotechnology advances

|December 1, 2025

概括

双子病毒是多功能DNA病毒,作为植物基因沉默,蛋白质表达和基因组编辑的载体. 它们的独特特性使得它们在合成生物学和作物改良领域的创新应用成为可能.

科学领域:

植物病毒学植物病毒学
分子生物学分子生物学
合成生物学合成生物学

背景情况:

双子病毒是一个大型的植物DNA病毒家族,在全球范围内引起了重要的作物疾病.
像茎环结构和复制蛋白 (Rep) 这样的关键特征允许功能双胞胎病毒复制品 (GVR).
30多年来,双子病毒一直被用作各种基因工程应用的载体.

研究的目的:

审查基因组结构,Rep蛋白功能,以及双胞胎病毒的历史应用.
突出双子病毒在病毒诱导基因沉默 (VIGS) 和合成生物学中的突出作用.
提供关于基于geminivirus的载体未来发展和潜在应用的前景.

主要方法:

审查关于双子病毒基因组学,复制和矢量应用的现有文献.
对VIGS,蛋白质表达和基因组编辑的双子病毒机制的分析.
探索基因调节元件和潜在的跨王国应用.

主要成果:

双子病毒载体通过基因替代或卫星DNA集成促进VIGS.
GVRs能够实现高水平的蛋白质表达,并提高CRISPR/Cas基因组编辑效率.
双子座衍生的调节元素扩大了植物的基因工程能力.

结论:

双子病毒载体是强大的工具,可以将病毒研究与作物改进和合成生物学联系起来.
未来的发展可能包括跨王国的应用,资源优化和工程双胞胎病毒作为"植物疫苗".
GVR为各种植物合成生物学设计提供了多功能,类似等离子体的DNA系统.

关键词:

这就是CRISPR/CasPR.双胞胎病毒复制体 (GVR)双胞胎病毒载体双胞胎病毒载体替代蛋白质是代表蛋白质的一种.合成生物学合成生物学病毒诱导的基因沉默 (VlGS)

更多相关视频

DNA Vector-based RNA Interference to Study Gene Function in Cancer

DNA Vector-based RNA Interference to Study Gene Function in Cancer

Published on: June 4, 2012

VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance

VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance

Published on: August 23, 2013

相关概念视频

In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

Genetic Screens

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...

Synthetic Biology

Synthetic Biology

Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...

siRNA - Small Interfering RNAs

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...

Microorganisms in Medicine and Therapeutics

Microorganisms in Medicine and Therapeutics

Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.