益生菌的闪光纳米封装:增强环境抵抗力和肠道殖民,以防止死性肠球炎
Yanbing Huang1, Weifan Niu2, Yuanbin He1
1Department of Pediatric Surgery, Fujian Children's Hospital (Fujian Branch of Shanghai Children's Medical Center), College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fuzhou 350014, PR China; Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou 350014, PR China.
概括
闪光纳米封装 (FNE) 改善了益生菌的传递,以预防死性肠球炎 (NEC). 封装的Lactobacillus rhamnosus GG (LGG) 显示在新生小鼠模型中增强了肠道存活率并减少了炎症.
科学领域:
- 生物技术是生物技术.
- 微生物学 微生物学
- 新生儿医学 新生儿医学
背景情况:
- 死性肠球炎 (NEC) 是一种具有高死亡率的新生儿胃肠急症.
- 益生菌可以预防NEC,但口服药物面临着可行性和殖民化的挑战.
- 纳米封装提供了一个解决方案,但目前的方法复杂且难以扩展.
研究的目的:
- 开发一种使用闪光纳米封装 (FNE) 技术的益生菌的通用纳米封装策略.
- 为了提高口服生物可用性和粘液粘合性Lactobacillus rhamnosus GG (LGG).
- 评估封装LGG在新生儿小鼠模型中预防NEC的疗效.
主要方法:
- 开创了闪光纳米封装 (FNE) 用于单细胞纳米封装各种益生菌菌株.
- 开发了用于Lactobacillus rhamnosus GG (LGG) 的多电解质-脂质双层封装.
- 在新生儿NEC小鼠模型中评估LGG疗效,分析肠道炎症,屏障功能和微生物群.
主要成果:
- 使用FNE实现了高效的LGG单细胞纳米封装.
- 显著提高了封装LGG的粘液粘性和口服生物可用性.
- 封装LGG通过TLR4/NF-κB通路缓解了肠道炎症,改善了屏障功能,并调节了NEC小鼠的肠道微生物群.
结论:
- FNE为益生菌纳米封装提供了一个可扩展和高效的方法.
- 封装LGG显示了预防新生儿NEC的显著治疗潜力.
- 这项工作为预防NEC的FNE基益生菌疗法奠定了基础.
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