隔离了一种Trichoderma asperellum突变的高水平β-葡萄糖酶活性,并用于林氏纤维素生物转化应用
Lu Mou1, Runze Pan1, Lianjie Wang1
1State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, PR China.
Bioresource technology
|December 1, 2025
概括
研究人员开发了一种超细胞分解的Trichoderma asperellum突变ML02,增强β-葡萄糖酶 (BGL) 活性,以有效分解葡萄糖. 使用这种突变的微生物联盟从生物质中获得了高乳酸产量,展示了具有成本效益的生物提炼潜力.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 生物化学 生物化学
背景情况:
- 均衡的酶复合物对于林氏纤维素降解至关重要.
- 低β-葡萄糖酶 (BGL) 活性限制了Trichoderma物种的纤维素水解效率.
研究的目的:
- 为了分离一个高细胞解 Trichoderma asperellum 突变,增强了 BGL 活性.
- 调查有助于改善细胞质活性和细胞质降解的因素.
- 评估微生物联盟在从纤维素酸生产乳酸 (LA) 的潜力.
主要方法:
- 乙基甲硫酸盐 (EMS) 突变发生和双板查被用于隔离突变物.
- 为了表型表征,进行了基因组和转录组分析.
- 一个由T. asperellum ML02和Lactobacillus paracasei组成的微生物联盟被构建用于LA生产.
主要成果:
- 一种T. asperellum突变ML02的BGL活性增加了2倍被成功分离出来.
- 超分支形态,胞缺陷和细胞壁完整性受损有助于增强细胞酶活性.
- 微生物联盟从Avicel获得了72.65g/L的LA产量,其纤维素降解效率为90%.
- 该财团从脱的米草中生产了60.74g/L的LA,这是生产菌素LA的创纪录.
结论:
- 突变T. asperellum ML02对具有成本效益的菌纤维素生物提炼具有显著的潜力.
- 开发的微生物联盟在将纤维素酸转化为乳酸方面表现出高效率.
- 这项研究为可持续的生物质利用提供了一个有希望的微生物平台.
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