多组学分析确定COX6A1和NAL调节剂是Plutella xylostella中丝形成的调节剂
Rui-Chang Niu1, Qing-Hui Zeng1, Wen-Jing Wang1
1State Key Laboratory of Crop Stress Biology for Arid Areas, Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs, College of Plant Protection, Northwest A&F University, Yangling 712100, China.
Pesticide biochemistry and physiology
|December 1, 2025
概括
钻石背的丝生产依赖于FibH,FibL和P25. 新的研究确定了COX6A1和NAL作为关键的调节剂,为十字植物作物提供可持续的害虫控制目标.
科学领域:
- 农业昆虫学 农业昆虫学
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 钻石背 (DBM) 是十字植物作物的重要全球性害虫.
- 在DBM幼虫中,丝的生产对于运动和化至关重要,由纤维素重链 (FibH),纤维素轻链 (FibL) 和纤维素素 (P25) 调节.
- 了解控制DBM丝生产的监管网络和下游基因对于开发有效的控制策略至关重要.
研究的目的:
- 调查参与钻石背丝生产的调节机制和下游基因.
- 通过分析丝基因缺失的影响来确定丝形成的关键调节者.
- 探索可持续害虫防治的新目标.
主要方法:
- 对CRISPR/Cas9产生的突变体进行PxFibH,PxFibL和PxP25的综合转录组和蛋白组分析.
- 不同基因表达 (DEG) 和蛋白质表达 (DEP) 分析.
- 基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 路径丰富分析.
- 对已识别的调节基因 (PxCOX6A1,PxNAL) 的CRISPR/Cas9淘汰试验.
主要成果:
- 转录组分析揭示了突变体中大量的DEGs,富含氧化减氧,跨膜传输和ECM受体相互作用通路.
- 蛋白质组分析确定了DEP参与能量代谢,酶活性和催化活性.
- 综合的多组基因发现了三个保存的调节基因:COX6A1,NAL和PPP1R14B.
- 淘汰PxCOX6A1导致子不完整,幼虫死亡率增加,卵位减少.
- PxNAL淘汰赛是致命的,异构卵体的形成,幼体重和生育能力都降低了.
结论:
- 在DBM中的丝生产取决于FibH/FibL/P25和相关的代谢网络.
- 细胞染色体c氧化酶子单元6A1 (COX6A1) 和N-乙烯基神经胺酸酶 (NAL) 被确定为DBM丝生产的新型,保存调节剂.
- COX6A1和NAL代表了开发对钻石背的环境可持续的害虫控制战略的有希望的目标.
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