齐多福维尔通过调节丝虫的多种免疫路径来诱导对Bombyx mori核聚合体病毒的抗病毒免疫力
Nasir Mehmood1, Mian Muhammad Awais1, Guangyu Ma1
1Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, Guangdong Engineering Technology Research Center of Sericulture, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
齐多福维尔有效地抑制了丝虫中的Bombyx mori核多重体病毒 (BmNPV) 复制. 这种抗病毒药物还可以增强先天免疫力和抗氧化酶活性,显示出对BmNPV控制的潜力.
科学领域:
- 病毒学 病毒学
- 昆虫病理学 昆虫病理学
- 免疫学 免疫学 免疫学
背景情况:
- 博姆比克斯·莫里核多重体病毒 (BmNPV) 在养殖中造成重大经济损失.
- 现有的BmNPV治疗方法无效,需要新的控制策略.
研究的目的:
- 评估Cidofovir对丝虫和BmN细胞中的BmNPV的抗病毒功效.
- 研究cidofovir对宿主免疫反应和肠道微生物群的影响.
主要方法:
- 细胞毒性测试以确定最佳的基多福维尔度.
- 西部涂抹和qRT-PCR用于评估病毒蛋白和DNA水平.
- 免疫相关基因和抗菌素的基因表达分析.
- 酶活性测定免疫和抗氧化剂标记物.
- 用于肠道微生物群分析的16S rRNA测序.
主要成果:
- 奇多福维尔显著降低了细胞和丝虫组织中的BmNPV蛋白VP39和病毒DNA负载.
- 观察到免疫基因 (Relish, STAT, Ago2) 和抗菌 (Cec-A, Cec-B) 的升级.
- 在接受治疗的丝虫中,增强了氧化酶,超氧化物脱酶和酶的活性.
- 在BmNPV感染和Cidofovir治疗后检测到肠道微生物组合的变化.
结论:
- 齐多福维尔有效抑制了BmNPV的复制.
- 齐多福维尔激活了丝虫中的多种先天免疫和抗氧化途径.
- 齐多福维尔显示出作为一种抗病毒药物,在丝养殖中对BmNPV的管理具有显著的潜力.
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