使用培养'omics探索微生物结构和功能在马体体内消化模型中的微生物结构和功能
Simon Daniels1, Susan Martin2, Pat Harris3
1Royal Agricultural University, Stroud Road, Cirencester, UK. simon.daniels@rau.ac.uk.
Scientific reports
|December 1, 2025
概括
马体内体外气体生产系统 (GPS) 显示了与捐赠体便相似的终点代谢特征,但更倾向于肠微生物而不是马体肠道细菌,表明潜在的功能冗余.
科学领域:
- 马的营养和微生物学
- 在体外发酵系统的发酵系统.
- 动物与马匹的消化系统生理学
背景情况:
- 实验室气体生产系统 (GPS) 已建立用于反动物料的分析.
- 适应GPS用于马类研究需要对其微生物和代谢行为进行表征.
- 了解马的肠道微生物组动态对于优化营养至关重要.
研究的目的:
- 在GPS中对马便注射剂的细菌群体和代谢体进行表征.
- 将GPS中的微生物和代谢资料与供体马便进行比较.
- 评估GPS适用于马类营养研究的适用性.
主要方法:
- 六只同样饮食的威尔士小马作为便捐赠者.
- 气体产量测量时间为156小时.
- 在便和注射液样本上进行了细菌群体概况 (16S rRNA 测序) 和代谢学 (1H NMR) 分析.
- 活力PCR (PMAxx) 评估了微生物的活力.
主要成果:
- 随着时间的推移,在GPS中观察到细菌社区形状和代谢表型的显著变化.
- 8小时发生了一种短暂的系统失调.
- 在GPS中的终点代谢概况类似于捐赠者便,但纤维降解细菌更符合肠,而不是马类文献.
- GPS准确地估计了干物质的消化能力.
结论:
- 马类GPS提供了与捐赠体便相似的终点代谢特征,并准确地估计了消化能力.
- 该系统可能有利于与体相关的微生物或与原生马肠道微生物群相比表现出功能冗余.
- 需要进一步的研究,以充分验证GPS用于马类应用.
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