综合性批量和单细胞RNA测序揭示了治疗性介质干细胞中的衰老相关生物标志物

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Emese Pekker^1,2, Erda Qorri^1,3, Márton Zs Enyedi^4,5

¹HCEMM-HUN-REN BRC Mutagenesis and Carcinogenesis Research Group, Institute of Genetics, HUN-REN Biological Research Centre, Szeged, Hungary.

Scientific reports

|December 1, 2025

概括

狗间酶干细胞 (MSC) 经历衰老,衰老过程减少了它们的治疗潜力. 这项研究描述了犬类MSC的衰老,揭示了关键的分子和转录变化.

科学领域:

细胞生物学细胞生物学
干细胞研究干细胞研究
老年学是一门学科.

背景情况:

介质细胞干细胞 (MSC) 对于细胞疗法至关重要,但由于衰老而导致重复分裂,它们失去了有效性.
狗模型为人类健康相关的衰老过程提供了翻译性见解.

研究的目的:

为了全面描述犬类介质干细胞 (MSC) 中的衰老特征.
为了识别与犬类MSC衰老相关的分子和转录特征.

主要方法:

对早期 (P2) 和晚期 (P6) 的犬类MSC进行比较分析.
利用与衰老相关的β-galactosidase (SA-β-gal) 染色和细胞循环造型.
用大量和单细胞RNA测序进行转录组分析.

主要成果:

晚期通道犬类MSCs表现出形态变化和SA-β-gal活性增加,表明衰老.
转录组分析显示,衰老标志物的显著上调和增殖基因的下调.
单细胞RNA测序确定了异构的衰老亚种群,并突出了衰老相关的分泌表型 (SASP) 基因上调.

结论:

穿越数显著影响狗的MSC特征,诱导衰老类型的表型.
综合的多组学方法,包括单细胞RNA测序,增强了临床应用的MSCs的识别和质量控制.

关键词:

动物模型动物模型生物标志物生物标志物介质细胞干细胞介质细胞干细胞.衰老是一种衰老.文字转录学 (Transcriptomics) 是一个学科.

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相关概念视频

Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...