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膨胀奥米克:从膨胀显微镜到空间奥米克

Zhen Dong^1,2,3, Weirong Xiang^4,5,6, Wenhao Jiang^4,5,6

¹Affiliated Hangzhou First People's Hospital, State Key Laboratory of Medical Proteomics, School of Medicine, Westlake University, Hangzhou, Zhejiang Province, China. dongzhen@westlake.edu.cn.

Molecular systems biology

|December 1, 2025

概括

扩展欧米克 (ExO) 使用物理组织扩张进行高分辨率的空间欧米克分析. 这项技术能够在多个omics层中精确地检测和绘制生物分子,从而推进空间生物学和医学.

科学领域:

空间生物学空间生物学
分子成像学分子成像学
奥米克斯技术的技术.

背景情况:

组织扩张 (ExO) 是一种最初用于超高分辨率成像的技术.
现在,ExO已经发展成为空间解析的omics分析的基本方法.
它可以精确检测生物分子,并将它们与生物功能联系起来.

研究的目的:

探索扩展omics如何整合多omics数据.
突出化学定策略在ExO中的作用.
讨论ExO在空间分辨率方面取得的进展.

主要方法:

利用物理组织扩张来提高空间分辨率.
采用化学定策略来进行选择性分子保留.
应用ExO在各种空间奥米克模式中,如表观遗传学,转录遗传学,蛋白质遗传学和脂质遗传学.

主要成果:

实现了用于omics分析的亚细胞分辨率和用于成像的亚纳米尺度.
启用了染色体状态,基因表达,蛋白质定位和脂质分布的高分辨率映射.
促进了空间多态学方法,以关联多个生物分子维度.

结论:

扩展omics是整个幻灯片,单细胞多omics的核心技术.
ExO将超高分辨率成像与空间奥米克集成在一起,以获得先进的生物洞察力.
需要进一步开发以应对人工智能虚拟细胞的分辨率,分子保留和数据分析方面的挑战.

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