聚氨酸酸酶B促进了醇的代谢转化为醇-3-乳酸
Hiroaki Konishi1, Ryo Matsui2, Tomomi Okamura2
1Yakult Central Institute, 5-11 Izumi, Kunitachi-Shi, Tokyo, Japan. hiroaki-konishi@yakult.co.jp.
Applied microbiology and biotechnology
|December 1, 2025
概括
聚氨酸酸酶B (PSP) 和酸合成酶α链 (TrpA) 是肠道微生物转化醇到醇-3-乳酸 (ILA) 中的关键酶. 协生菌增强了这个过程,提供了潜在的健康益处.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 肠道微生物组研究研究
背景情况:
- 肠道细菌将英多尔代谢成各种化合物,但酶途径尚未完全理解.
- 某些Bifidobacterium菌株使用特定的酶将醇转化为醇-3-乳酸 (ILA).
- 协生菌,结合细菌和寡糖,增强了醇代谢,但机制尚不清楚.
研究的目的:
- 为了研究由同生菌增强的醇代谢背后的酶机制.
- 识别关键的微生物酶,参与转化英多尔到ILA.
- 阐明素酸酶B (PSP) 和酸盐合成酶α链 (TrpA) 在这一途径中的作用.
主要方法:
- 在Bifidobacterium bifidum YIT 10347中利用Tn5转位子突变发生,以选在内代谢中必需的基因.
- 在野生类型和突变菌株中评估的内醇转化为ILA的转化率.
- 研究了血清蛋白补充剂对PSP缺陷突变物中的ILA产生的影响.
主要成果:
- 缺乏PSP (编码为serB) 或TrpA (编码为trpA) 的突变菌株显著减少了ILA的产生.
- 比菲多细菌 (Bifidobacterium bifidum) YIT 10347结合银河藻类糖 (GOS) 协同增强的醇转化为ILA.
- 血清素补充恢复了PSP缺乏菌株的ILA生产,突出了血清素生物合成的作用.
结论:
- PSP和TrpA是微生物代谢中的关键酶,用于将内醇转化为ILA.
- 协生菌,特别是B. bifidum YIT 10347和GOS,显著增强了醇代谢.
- 这一途径为肠道和脏健康提供了潜在的治疗应用.
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