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一种新型酵母Cyberlindnera fabianii的生物表面活性剂生产MIAU-1:过程优化,结构特征和功能分析

Kubra Eryasar-Orer¹, Seda Karasu-Yalcin², Elif Berna Olutas³

¹Department of Food Engineering, Faculty of Engineering, Bolu Abant Izzet Baysal University, 14030, Bolu, Türkiye.

International microbiology : the official journal of the Spanish Society for Microbiology

|December 1, 2025

概括

一种新型酵母,Cyberlindnera fabianii MIAU-1,利用葡萄糖和向日油有效地产生脂生物表面活性剂. 这种生物表面活性剂表现出强大的抗微生物和抗粘性质,以及稳定的表面活性.

科学领域:

微生物学和生物技术
生物化学和有机化学
材料科学材料科学材料科学

背景情况:

生物活性剂是两性化合物,具有多种应用,但它们的高效和成本效益的生产仍然是一个挑战.
酵母物种,特别是非传统的酵母物种,越来越多地被探索它们在生物表面活性剂合成中的潜力.
赛博林德纳 (Cyberlindnera fabianii) 是一种酵母属,对生物表面活性剂生产能力的探索有限.

研究的目的:

为了研究新型Cyberlindnera fabianii MIAU-1隔离物的生物表面活性剂生产潜力.
为了优化发酵条件以提高生物表面活性剂产量.
描述产生的生物表面活性剂并评估其功能性质,包括表面活性,稳定性和生物活性.

主要方法:

使用碳基板 (葡萄糖和向日油) 和源 (酵母提取物和尿素) 来发酵Cyberlindnera fabianii MIAU-1.
在震动瓶和生物反应器系统中优化基质度和发酵时间.
使用FT-IR,NMR和热重力学分析 (TGA) 净化和表征生物表面活性剂.
评估表面张力减小,临界菌度 (CMC),表面活动稳定性,抗菌活性和抗粘性质.

主要成果:

在最佳发酵条件下,生物反应器在48小时内产生了39.85g/L的生物表面活性剂,这与摇瓶生产相比显著改善.
生产的生物表面活性剂被确定为一种甘油脂 (脂),降解温度为255°C (原始) 和275°C (纯化).
原始生物表面活性剂表现出有效的表面张力降低 (40mN/m在43.63 mg/LCMC) 和在各种条件下稳定的活性.
该生物表面活性剂对Listeria monocytogenes,Salmonella Enteritidis和Escherichia coli O157:H7表现出显著的抗菌活性,并抑制了Staphylococcus aureus和L. monocytogenes生物膜的形成.

结论:

网络lindnera fabianii MIAU-1 是一个有前途的候选人,用于高效的脂生物表面活性剂生产.
生产的生物表面活性剂具有有价值的功能性质,包括卓越的表面活性,稳定性和抗微生物/抗粘合能力.
这项研究强调了酵母衍生生物表面活性剂在各种工业和生物医学应用中作为可持续替代品的潜力.

关键词:

网络监视者法比安尼具有抗粘性活性.具有抗菌活性,具有抗菌活性.批量发酵是一种批量发酵.生物表面活性剂是生物表面活性剂.

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