相关实验视频

Updated: Jan 9, 2026

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer

Published on: March 14, 2019

循环的microRNA签名用于球菌病

Rui Li^1,2, Xuedong He², Shuangjuan Liu³

¹College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, 730070, China.

Parasites & vectors

|December 2, 2025

概括

循环中的微RNAs (miRNAs) 显示出用于诊断 Echinococcosis 的前景. 肝脏特定的miRNAs,miR-192-5p和miR-122-5p,在感染期间下调,并随着治疗而增加,有助于诊断.

科学领域:

寄生虫学的寄生虫学
分子生物学分子生物学
生物标志物发现发现

背景情况:

脊髓灰质炎是一种严重的动物性寄生虫病,具有很长的潜伏期.
准确的诊断对于有效的患者管理和治疗至关重要.
识别可靠的诊断标志物对于球菌病是必不可少的.

研究的目的:

为了确定球菌病的强有力的诊断特征.
在感染宿主中分析阿尔戈诺特2结合微RNA (abmiRNAs).
开发和验证基于这些abmiRNAs的诊断试验.

主要方法:

同免疫沉和RNA测序 (RNA-seq) 用于在小鼠肝细胞中分析abmiRNAs.
定量PCR (qPCR) 试验是基于循环中的肝脏特异性abmiRNAs.
通过使用感染小鼠和人类的血清样本验证了测试结果.

主要成果:

确定了三个肝脏特异性的abmiRNAs (miR-192-5p,miR-122-5p,miR-21a-5p).
循环中的abmiRNAs在感染期间显著下调,在治疗后上调.
qPCR测定在区分感染方面表现出高灵敏度和特异性,miR-192-5p和miR-122-5p组合特别有效.

结论:

循环中的miR-192-5p和miR-122-5p作为血清生物标志物用于球菌病诊断.
这些miRNAs可以帮助预后管理疾病.
鉴定的生物标志物显示出非侵入性诊断和治疗反应监测的潜力.

关键词:

埃奇诺科克多部位球菌是多部位球菌.诊断和预后签名的诊断和预后签名.埃奇诺可可菌病 (echinococcosis) 是一种可怕的疾病.在米R-122-5p.在 MiR-192-5p 里面.在米R-21a-5p.

更多相关视频

MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier MSC for Lung Cancer Screening

MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier MSC for Lung Cancer Screening

Published on: October 26, 2017

Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile

Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile

Published on: June 13, 2016

相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...