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单细胞转录组分析阐明了结直肠癌和肉类症之间的微环境相互作用

Tihong Qiu¹, Xiaoying Wang², Jie Li¹

¹Department of Gastrointestinal Surgery, The Third Affiliated Hospital of Chengdu Medical College, Pidu District People's Hospital, Chengdu, China.

|December 2, 2025

概括

结肠直肠癌和肉症共享共同的细胞类型,但表现出不同的功能. 这项研究揭示了跨组织的细胞异质性和潜在的调节者,为瘤系统效应和肉类病机制提供了新的见解.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
老年学是一门学科.

背景情况:

结肠直肠癌 (CRC) 经常与肉症 (SP) 一起发生,影响患者的预后.
驱动这种相互作用的组织病理机制在很大程度上是未知的.
以前的研究缺乏跨组织微环境相互作用的直接分子证据.

研究的目的:

研究结直肠癌和肉类症之间的分子机制和跨组织相互作用.
解决CRC和SP之间的共享细胞类型中的细胞异质性和功能差异化.
为了确定潜在的调节者参与瘤和肉类的系统性影响.

主要方法:

综合分析来自16个CRC组织和17个SP组织的单细胞RNA测序 (scRNA-seq) 数据.
使用Harmony进行批量效果校正和组合细胞注释.
采用差异表达分析和CellChat通信建模.

主要成果:

在CRC和SP之间确定了共享的核心细胞类型 (内皮细胞,纤维细胞,单细胞),具有不同的转录形状.
CRC细胞在炎症性,血管性和矩阵重塑途径中表现出丰富,与缺氧相关.
脊髓炎细胞倾向于肌肉维护,代谢调节和修复功能.
在每个条件中确定了特定的活性通路 (MIF-CD74,COLLAGEN,THBS-CD47在CRC中;SDC4,SP中的CD36).
发现了VWF,PDGFRA和FCN1的稳定表达,这表明一个保存的平稳网络.

结论:

这项研究阐明了CRC和SP中共享细胞类型的跨组织功能异质性.
确定了对观察到的相互作用有助于潜在的普遍调节者.
为了解瘤系统效应和萨尔科佩尼亚的微环境机制提供了新的视角.

关键词:

结肠直肠癌是什么意思代谢异质性的代谢异质性微环境重编程微环境重编程这种类型的麻症是sarcopenia.一个单细胞转录组的转录组.

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