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在Loxostege sticticalis中对尿路发育的光周期调节:协调的20-基松动态和PTTH/EcR/USP动态

Jin Cui¹, YueQiu Liu², XiaoFei Lv¹

¹State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.

Pest management science

|December 2, 2025

概括

光周期通过控制PTTH-20E-EcR/USP轴来调节甜菜网虫的隔离. 这一途径涉及20-hydroxyecdysone (20E),决定了在断和非断状态中的发育过渡.

科学领域:

昆虫学昆虫学是一门学科.
昆虫生理学昆虫生理学
分子生物学分子生物学

背景情况:

甜菜网虫 (Loxostege sticticalis) 是一个重要的农业害虫,它以成熟的幼虫的形式在隔离期过冬.
光周期是一个关键的环境线索,影响昆虫的发育和断.
光周期-20-基乙 (20E) 轴在该物种中调节幼虫隔离的确切作用尚不清楚.

研究的目的:

阐明光周期-20-氧乙 (20E) 轴在甜菜网虫中调节隔离的作用.
了解光周期如何影响控制发育过渡的荷尔蒙通路.

主要方法:

在不同光周期下对20E标位和基因表达 (PTTH,Ecr,USP) 的分析.
使用20E激动剂微注射和RNAi介导基因敲除的功能验证.

主要成果:

短时间的光周期抑制了PTTH表达,并降低了EcR/USP的调节,维持了隔离期,而20E水平波动.
长日光周期导致20E,PTTH,Ecr和USP的增加,促进了发展.
20E激动剂加速了化,而对PTTH,EcR或USP的抑制延长了幼虫的发育,证实了它们的作用.

结论:

光周期通过PTTH抑制破坏20E生物合成,导致Ecr/USP沉默,从而维持隔膜.
PTTH-20E-EcR/USP轴在甜菜网虫中是发展过渡的关键调节者,在半径和非半径状态中进行过渡.

关键词:

20-基乙基迪松 20-基乙基迪松洛克索斯塔格 (Loxostege) 是一种粘性质的粘性质.在PTTH/EcR/USP中使用.这是一种RNA干扰.调节调节调节调节调节调节激素激动剂激素激动剂

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