稳定了细菌离子通道DeCLICIC的N端灵活域
Chen Fan1,2,3, Marie Lycksell2, Yuxuan Zhuang2
1Dept. of Applied Physics, Science for Life Laboratory, KTH Royal Institute of Technology, Solna, Sweden.
研究人员研究了对敏感通道DeCLIC,揭示了其N端域 (NTD) 如何影响离子通道功能. 结合稳定了NTD,影响了通道关,并提供了对灵活域角色的洞察力.
科学领域:
- 生物物理学的生物物理.
- 结构生物学 结构生物学
- 神经科学是一个神经科学.
背景情况:
- 米联离子通道 (pLGICs) 介导快速的化学到电信号传导.
- 一些 prokaryotic pLGIC 具有一个 N-终端域 (NTD),其结构和功能未被定义.
- 敏感通道DeCLIC的NTD可能会加速门,但其灵活性使结构分析复杂化,其在敏感性中的作用尚不清楚.
研究的目的:
- 阐明DeCLIC N-终端域 (NTD) 的结构和功能.
- 确定在DeCLIC通道调制中的作用.
- 为理解灵活的辅助域参与离子通道活动提供结构基础.
主要方法:
- 在脂质纳米盘中使用DeCLIC的冷电子显微镜 (cryo-EM).
- 高分辨率的结构确定.
- 分子动力学 (MD) 模拟.分子动力学 (MD) 模拟.
主要成果:
- 实现了DeCLIC的最高分辨率的冷电磁结构.
- 在NTD和细胞外域中确定结合部位.
- 观察到耗诱导的不对称NTD形状,与先前的散射数据一致.
- MD模拟证实了NTD的介导稳定.
结论:
- 德克利克的NTD在敏感性和离子通道关中起着至关重要的作用.
- 结合稳定了NTD,影响了通道的形状.
- DeCLIC作为一种模型,用于理解离子通道中的灵活辅助域调制,在整个进化过程中可能被保存.
更多相关视频
12:26Author Spotlight: Functional Site-Directed Fluorometry in Native Cells to Study Skeletal Muscle Excitability
Published on: June 2, 2023
08:27Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy
Published on: January 7, 2019
相关概念视频
Calmodulin-dependent Signaling
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
Ligand-Gated Ion Channel Receptor: Gating Mechanism
Feedback Regulation of Calcium Concentration
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
Structure of Cadherins
Ligand-gated Ion Channels
Three Subfamilies of Ligand-gated Ion Channels
Ligand-gated ion channels fall into three subfamilies. The 'Cys-loop' includes the nicotinic acetylcholine receptors, γ-aminobutyric acid (GABA), glycine, and 5-hydroxytryptamine receptors. The second one is the 'Pore-loop' channels that...
The Role of Ion Channels in Neuronal Computation
Sometimes a single EPSP is strong enough to induce an action potential in the postsynaptic neuron. However, multiple presynaptic inputs must often create EPSPs around the same time for the postsynaptic neuron to be sufficiently depolarized to fire an action potential....
