在口腔状细胞癌进展中,TNF-α和自之间的相互调节
Chih-Wen Shu1,2,3, Chun-Feng Chen4, Chia-Che Chang5
1Institute of BioPharmaceutical Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan.
概括
瘤坏死因子-α (TNF-α) 和自会影响口腔状细胞癌 (OSCC) 的进展. 这项研究揭示了TNF-α和自之间的相互调节,影响OSCC细胞活力和患者存活率.
科学领域:
- 在瘤学瘤学.
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 自和瘤亡因子-α (TNF-α) 在口腔状细胞癌 (OSCC) 的发展中起着关键作用.
- 在OSCC进展中自和TNF-α之间的复杂关系尚未完全理解.
研究的目的:
- 在OSCC进展的背景下调查自和TNF-α之间的相互作用.
- 阐明TNF-α在OSCC中影响自和相反的机制.
主要方法:
- 细胞因子水平使用生物复合试验量化.
- 自激活通过共聚焦显微镜和特定基因淘汰来评估.
- 细胞活力,生长和NF-κB p65活性使用各种测试进行了评估,包括CellTiter-Glo,克隆基因测试和记者测试.
- 分析了OSCC患者的临床数据,以将分子发现与生存结果相关联.
主要成果:
- TNF-α水平与自调节有复杂的关系,随着自抑制而下降,并在自诱导条件下增加.
- 暴露于TNF-α促进了OSCC细胞活力和自,而自抑制剂可以逆转这些影响.
- 与癌前疾病相比,OSCC患者观察到血清TNF-α水平升高,并且与疾病特异性存活率较差有关.
- 在RelA,LC3和P62表达之间发现了积极的相关性,TNF-α与自标志物 (P62,LC3) 和RelA的联合表达与较短的无病生存时间有关.
结论:
- 在TNF-α和自之间存在一个相互调节循环,对OSCC的进展有显著的贡献.
- 针对TNF-α和自之间的相互作用,为OSCC提供了一个潜在的治疗策略.
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