Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

8.1K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
8.1K
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

6.4K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
6.4K
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

2.3K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
2.3K
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

1.5K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
1.5K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Native Charge Detection Mass Spectrometry of Kilobase-Scale Messenger RNAs.

Analytical chemistry·2026
Same author

Temperature and lipid composition differentially regulate KRAS assemblies on membranes.

Chemical communications (Cambridge, England)·2026
Same author

Time-Resolved Native Mass Spectrometry for Direct Measurement of Biomolecular Kinetics.

Journal of the American Chemical Society·2026
Same author

Multivalent recognition of ferritin by full-length NCOA4 enables robust ferritinophagy.

Protein science : a publication of the Protein Society·2026
Same author

Peptidomimetic α,β-Unsaturated Ethyl Esters Are Irreversible Inactivators of Human Cathepsin L and Are Potent Inhibitors of SARS-CoV-2 in Cellular Models of COVID-19.

Journal of medicinal chemistry·2026
Same author

Rubisco kinetic acclimation at the holoenzyme level.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Strain-Level Food Surveillance of <i>Escherichia coli</i> Using a Specific-Nonspecific Hybrid Sensor Array Strategy.

Analytical chemistry·2026
Same journal

A Field-Portable Fe(IV)-Mediated Competitive Quenching Chemiluminescence Platform with a Synchronous Y-Shaped Flow-through Cell for Broad-Spectrum Quantification of Volatile Phenols.

Analytical chemistry·2026
Same journal

Single-Molecule Characterization of CRISPR-Cas12a for Amplification-Free Genetic Testing.

Analytical chemistry·2026
Same journal

Integrated Acoustofluidic Manipulation and Oscillation-Stabilized Magnetic Relaxation Biosensing for <i>Salmonella</i> Detection.

Analytical chemistry·2026
Same journal

A Self-Powered Sensing Platform Based on the Janus Heterostructure for Machine Learning-Assisted Dual-Mode Detection of 17β-Estradiol.

Analytical chemistry·2026
Same journal

Large Language Model-Generated Dietary Metabolite Biomarker Database Drives Deep Annotation of the Human Diet Metabolome.

Analytical chemistry·2026
查看所有相关文章

相关实验视频

Updated: Jan 9, 2026

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
10:01

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Published on: November 28, 2017

20.3K

使用直接质量技术剖析蛋白质复合样本的异质群体.

Robert L Rider1, Jared Hampton1, Zhenyu Xi1

  • 1Department of Chemistry, Texas A&M University, College Station, Texas 77843, United States.

Analytical chemistry
|December 2, 2025
PubMed
概括
此摘要是机器生成的。

电荷检测质谱 (CDMS) 现在可以对异质蛋白样本进行定量分析. 这种技术准确地测量了质量和丰度,推动了生物研究.

更多相关视频

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools
07:01

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools

Published on: August 19, 2025

773
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

12.6K

相关实验视频

Last Updated: Jan 9, 2026

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
10:01

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Published on: November 28, 2017

20.3K
Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools
07:01

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools

Published on: August 19, 2025

773
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

12.6K

科学领域:

  • 质谱测量质量谱测量
  • 生物物理化学 生物物理化学
  • 蛋白质组学是指蛋白质组学.

背景情况:

  • 电荷检测质谱 (CDMS) 是一种新兴的技术,用于对异质生物样本进行定性分析.
  • 基于轨道飞行器的CDMS增加了该技术在本地质谱 (nMS) 中的使用.
  • 目前CDMS的应用主要是定性性的,在MDa范围内进行的定量研究有限.

研究的目的:

  • 为了证明基于Orbitrap的CDMS/直接质量技术 (DMT) 对100kDa以下的异质蛋白样本的定量能力.
  • 分析使用DMT的跨甲状腺素 (TTR) 变体的稳定性和结合因子特性.
  • 建立CDMS作为复杂生物系统的可行的定量方法.

主要方法:

  • 利用基于轨道飞行器的CDMS/直接质量技术 (DMT) 进行高分辨率质量分析.
  • 已确定和量化野生类型 (WT) 和C端标记 (CT) 的TTR同位素和混合四位素.
  • 分析了混合TTR的动态形成,并量化了对TTR变体的甲状腺素结合.

主要成果:

  • DMT成功地解决了WT和CT TTR同位四度体和混合四度体,从而实现了动力分析.
  • 与WT-TTR相比,CT-TTR同位四聚体的稳定性下降,这表明C端修饰影响四聚体的稳定性.
  • DMT量化了甲状腺素结合,揭示CT-TTR的亲和力比WT-TTR更高,表明C端参与了连接体结合.
  • CDMS分离了重叠的m/z信号,使异质样本的质量和丰度能够准确地确定.

结论:

  • 基于Orbitrap的CDMS/DMT被确立为异质蛋白样本的定量方法.
  • TTR的C终端修饰影响了四聚体稳定性和甲状腺素结合亲和力.
  • 在分析复杂的生物系统方面,CDMS显示出更广泛应用的巨大潜力.