塞斯特林2通过LAT1/mTOR通路调节牛奶蛋白合成
Yongding Ke1,2, Binglan Chen2, Xinping Wang2
1College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, Shandong Province 266109, China.
Journal of agricultural and food chemistry
|December 2, 2025
概括
塞斯特林2 (SESN2) 通过与白蛋白载体LAT1.1相互作用来调节牛奶蛋白合成. 这项研究揭示了SESN2
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 动物科学动物科学
背景情况:
- 塞斯特林2 (SESN2) 是一种白蛋白传感器,它调节mTOR信号传递和蛋白质合成.
- 在乳腺功能和牛奶蛋白合成中SESN2的确切作用尚不清楚.
- 了解SESN2的机制对于改善哺乳期和奶牛遗传学至关重要.
研究的目的:
- 研究SESN2在牛乳表皮细胞 (MAC-T) 牛乳蛋白合成中的分子机制.
- 为了确认SESN2在小鼠乳腺中的哺乳调节功能.
- 阐明乳腺功能中SESN2和LAT1之间的交叉对话.
主要方法:
- 在MAC-T细胞中进行SESN2淘汰 (KD),在小鼠中进行SESN2淘汰 (KO).
- 对mTOR信号通路组件 (mTOR,S6K酸化) 的分析.
- 评估素表达 (α-和β-素) 和LAT1分布.
- 在小鼠乳腺扩展物中评估牛奶产量和白氨酸感应.
主要成果:
- 在MAC-T细胞中,SESN2 KD增加了mTOR/S6K酸化和素表达.
- SESN2 KD导致LAT1表达和分布在MAC-T细胞膜中增加.
- 在SESN2 KO小鼠中,牛奶产量增加和素的表达增加.
- 在LAT1抑制后,SESN2 KO小鼠表现出白蛋白感应受损和mTOR/S6K酸化降低.
结论:
- 这项研究证明了新型的SESN2-LAT1交叉谈话在调节牛奶蛋白质合成和哺乳期.
- 在乳腺功能和哺乳中,SESN2起着重要的作用.
- 这些发现为哺乳机制和乳牛遗传改进的潜在目标提供了新的见解.
相关概念视频
PI3K/mTOR/AKT Signaling Pathway
5.3K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
5.3K
mTOR Signaling and Cancer Progression
4.6K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
4.6K
Operons
53.9K
Prokaryotes can control gene expression through operons—DNA sequences consisting of regulatory elements and clustered, functionally related protein-coding genes. Operons use a single promoter sequence to initiate transcription of a gene cluster (i.e., a group of structural genes) into a single mRNA molecule. The terminator sequence ends transcription. An operator sequence, located between the promoter and structural genes, prohibits the operon’s transcriptional activity if bound by...
53.9K
Inducible Operons: lac Operon
1.3K
The lac operon in Escherichia coli is a model for understanding inducible gene regulation and metabolic flexibility. It integrates local control by lactose and global regulation through catabolite repression, enabling E. coli to preferentially metabolize glucose when available and switch to lactose utilization when glucose is scarce.Structure and Function of the lac OperonThe lac operon contains three structural genes: lacZ (β-galactosidase), lacY (lactose permease), and lacA...
1.3K
Regulation of Nuclear Protein Sorting
3.2K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
3.2K
Stringent Response in E. coli
267
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
267


