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富勒 C₆₀(OH) _22-24对人类外周血液单细胞的细胞毒性

V P Timganova¹, M S Bochkova^2,3, S S Lazarev²

¹Institute of Ecology and Genetics of Microorganisms, Perm Federal Research Center, Ural Branch, Russian Academy of Sciences, Perm, Russia. timganovavp@gmail.com.

Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections

|December 2, 2025

概括

富勒C60 ((OH) 22-24在高达100微克/毫升的度下对人类单细胞活力产生最小的影响. 高度降低了生命力,与纳米粒子吸收相关,表明了潜在的生物医学应用.

科学领域:

纳米医学是一种纳米医学.
免疫毒理学免疫毒理学
生物材料是一种生物材料.

背景情况:

富勒醇是水溶性富勒衍生物,具有潜在的生物医学应用.
了解它们与单细胞等免疫细胞的相互作用对于安全性评估至关重要.

研究的目的:

为了评估富勒C60(OH) 22-24对人类外周血液单细胞活性的影响.
研究单细胞对富勒纳米颗粒的粘附和内化.

主要方法:

人类外周血液单核细胞在不同度的富勒纳米颗粒 (0.25200μg/mL) 中进行了24小时,48小时和72小时的化.
使用流式细胞计量评估了单细胞活力.
通过测量单细胞门内的光强度来量化富勒醇吸收.

主要成果:

纳米粒子吸附和内部化随着度和化时间的增加而增加.
富勒C60(OH) 22-24在200微克/毫升下,在72小时内将单细胞活力降低了17-30%.
细胞存活率与纳米粒子吸收强度成反比例.

结论:

富勒醇度高达100μg/mL,即使在长时间暴露后,也没有对单细胞产生细胞毒性.
这些发现支持富勒纳米颗粒的潜在生物医学适用性.
单细胞与富勒伦醇的相互作用是度和时间依赖的.

关键词:

细胞毒性细胞毒性富勒 C60 ((OH) 2224) 是一种富勒.人类单细胞.纳米颗粒的内部化/粘附.纳米颗粒是一种纳米粒子.

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