circASbase:circRNAs中替代分离事件的综合数据库
Lingxiao Zou1, Jian Zhao1, Haojie Li2,3,4
1Department of Biomedical Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 211106, China.
Genomics, proteomics & bioinformatics
|December 2, 2025
概括
一个新的数据库,circASbase,对13种物种的45万多个循环RNA (circRNA) 替代拼接事件进行了目录. 该资源揭示了circRNAs中独特的拼接模式,影响了它们的功能和调节.
科学领域:
- 分子生物学分子生物学
- 生物信息学是一种生物信息学.
- 基因组学就是基因组学.
背景情况:
- 替代拼接产生各种RNA异型,包括循环RNA (circRNAs).
- 缺乏专门的数据库阻碍了研究circRNA替代拼接事件.
- 了解circRNA拼接对于它们的功能多样性至关重要.
研究的目的:
- 开发circASbase,这是一个针对circRNA替代拼接事件的全面数据库.
- 为研究circRNA拼接调节提供丰富的注释.
- 促进研究circRNA替代拼接的功能作用.
主要方法:
- 在884,047个全长circRNA中编目了452,129个替代拼接事件.
- 分析了来自13个物种的581个样本的数据.
- 开发用户友好的界面和基于Web的可视化工具.
主要成果:
- 在circRNAs和线性转录之间的替代拼接中发现了实质性的差异.
- 证明circRNA剪接事件会影响IRES站点,m6A站点,ORF和miRNA目标.
- 展示了独特的监管格局和circRNA拼接的功能影响.
结论:
- circASbase解决了对circRNA拼接数据库的需求.
- 该数据库促进了对circRNA生物学和调控机制的理解.
- circASbase是探索 circRNA 替代拼接和功能的宝贵资源.
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