Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Spatiotemporal Decoding of Explore-Exploit Decisions in the Human Brain.

bioRxiv : the preprint server for biology·2026
Same author

AI-Discovered Cognitive Models Reveal Novel Insights into Human and Animal Learning.

bioRxiv : the preprint server for biology·2026
Same author

Monitoring in vivo transcription with synthetic serum markers.

Nature communications·2026
Same author

Synthetic serum markers enable noninvasive monitoring of gene expression in primate brains.

Neuron·2026
Same author

Monitoring Gene Expression in Retina with synthetic serum markers.

bioRxiv : the preprint server for biology·2026
Same author

Site-specific noninvasive delivery of retrograde viral vectors to the brain.

Bioengineering & translational medicine·2026
Same journal

A predisposing effect of HLA class II genes in celiac disease by skewing the naive CD4<sup>+</sup> T cell receptor repertoire.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Wave propagation in fluid-saturated nanoporous media: Upscaling molecular mechanics into continuum-level description.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Collagen-producing eye cell atlas reveals distinct fibroblast fates in early injury vs. fibrotic subretinal disease.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Knotted solid tori in contact manifolds.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Biophysical fitness landscape design traps viral evolution.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Cryo-EM of the eukaryotic purine transporter UapA demonstrates intramolecular and lipid regulation of transport.

Proceedings of the National Academy of Sciences of the United States of America·2026
查看所有相关文章

相关实验视频

Updated: Jan 9, 2026

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay
08:22

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

10.1K

可删除的血清标志物

Shirin Nouraein1,2,3, Honghao Li1,2, Sangsin Lee1,2

  • 1Department of Bioengineering, Rice University, Houston, TX 77005.

Proceedings of the National Academy of Sciences of the United States of America
|December 2, 2025
PubMed
概括
此摘要是机器生成的。

研究人员开发了可删除的释放活性标记物 (RMAs),以非侵入性地监测大脑基因表达. 这种新方法显著减少了背景信号,改善了通过血液检测敏感检测神经活动的动态范围.

关键词:
基因表达的监测 基因表达的监测血清标志物的血清标志物时间分辨率时间分辨率

更多相关视频

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery
13:00

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery

Published on: April 17, 2012

13.9K
Western Blotting: Sample Preparation to Detection
07:45

Western Blotting: Sample Preparation to Detection

Published on: October 14, 2010

145.1K

相关实验视频

Last Updated: Jan 9, 2026

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay
08:22

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

10.1K
Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery
13:00

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery

Published on: April 17, 2012

13.9K
Western Blotting: Sample Preparation to Detection
07:45

Western Blotting: Sample Preparation to Detection

Published on: October 14, 2010

145.1K

科学领域:

  • 神经科学是一个神经科学.
  • 生物技术是生物技术.
  • 分子生物学分子生物学

背景情况:

  • 大脑基因表达分析通常需要侵入性方法,如活检或死后组织学.
  • 目前用于大脑监测的血清标记是有限的,缺乏特定的细胞定位,并且受到动态跟踪的长半衰期的影响.

研究的目的:

  • 开发一种使用血清标记器进行敏感和动态监测大脑基因表达的非侵入性方法.
  • 为了克服长血清半衰期的局限性,当前的血清标志物用于跟踪时间变化.

主要方法:

  • 为测量转基因表达而设计的"按需可删除的释放活性标记" (RMA).
  • 使用静脉注射向蛋白酶来减少RMA背景信号.
  • 验证了系统在检测小鼠中仅12个神经元的表达时的灵敏度.

主要成果:

  • 工程RMAs显示了>100小时的血清半衰期,并从最小的神经元群体中检测到表达.
  • 可删除的RMAs与蛋白质酶注射相结合,将背景信号减少了超过一个数量级.
  • 该系统实现了65,000倍的信号增强,并改善了用于检测低级别促进器活动的动态范围.

结论:

  • 可删除的RMA提供了一种灵敏而非侵入性的方法,通过简单的血液测试来监测大脑基因表达.
  • 这项技术增强了跟踪基因表达的动态范围,特别是对于与生理状态相关的低水平促进子活动,如c-Fos.
  • 开发的系统代表了研究大脑活动和基因调节的非侵入性研究的重大进步.