在堆肥中来自猪的ARG的迁移特征 - 土壤 - 菜
Yihao Huang1, Ruyu Li1, Yuzhen Dai1
1College of Animal Sciences & Technology/College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
Ecotoxicology and environmental safety
|December 2, 2025
概括
优化猪肥堆肥以更高的碳比率有效降低抗生素耐药性基因 (ARG). 然而,一些ARG会持续存在并扩散到土壤和植物,这凸显了需要谨慎管理的必要性.
科学领域:
- 环境微生物学 环境微生物学
- 农业科学 农业科学
- 公共卫生 公共卫生
背景情况:
- 抗生素耐药性基因 (ARG) 是一个日益严重的环境问题.
- 猪肥堆肥是一种常见的农业实践,有可能传播ARG.
- 在堆肥和随后的土壤植物转移期间了解ARG命运至关重要.
研究的目的:
- 调查101个ARG在有氧猪肥堆肥过程中的污染模式,持久性和命运.
- 评估ARG在受精后在土壤植物系统中传播的情况.
- 评估不同碳 (C/N) 比率对ARG减少和微生物群落结构的影响.
主要方法:
- 猪的有氧堆肥使用C/N比为15:1,25:1,35:1.
- 使用qPCR对101个ARG进行绝对量化.
- 用于微生物社区分析的元基因组测序.
- 在受肥土壤和生菜中分析ARG的丰度.
主要成果:
- 堆肥显著降低了四环素,化物和β-乳酸盐的ARG,而β-乳酸盐的降低率最高 (73-89%).
- 较高的C/N比率 (25:1, 35:1) 提高了ARG的减少.
- 堆肥改变的微生物群落,其中Luteimonas可能是持久性ARG和与可减少ARG相关的Acinetobacter的潜在宿主.
- ARG,特别是硫胺基因 (sul1, sul2),在受精土壤中增加,并在菜中检测到,在C/N 25:1组中土壤ARG的丰度较低.
结论:
- 调节堆肥C/N比率可以有效地减少ARG,但不能完全消除.
- 持久的ARG可以转移到土壤和植物,构成潜在的风险.
- 在堆肥过程中微生物社区的转移会影响ARG的命运,这表明有针对性的管理策略.
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