用于MALDI-2MS的臭氧化成像,在生物组织中对酸乙醇胺的碳-碳双键定位异构体进行成像
Dominika Bezdeková1, Michal Hendrych2, Jan Schwenzfeier3
1Department of Chemistry, Faculty of Science, Masaryk University, Brno, Czech Republic.
Analytica chimica acta
|December 2, 2025
概括
这项研究引入了MALDI-2激光后电离,以改善组织中酸乙烯胺 (PE) 的成像. 改进的方法成功地绘制了小鼠胚胎和人类癌症中的位置性脂质异构体,揭示了不同的空间分布和潜在的代谢作用.
科学领域:
- 利皮多米克 (Lipidomics) 是一种消化剂.
- 质谱仪成像成像 质谱仪成像
- 分子成像学分子成像学
背景情况:
- 甲基乙醇胺 (PE) 是关键的脂质,但由于臭氧化离子丰度低,通过MALDI MSI对它们的异构成像具有挑战性.
- 脱线组织上臭氧化,其次是MALDI MSI,使脂双键 (db) 定位异构体的空间分化成为可能.
研究的目的:
- 用MALDI与激光后电离 (MALDI-2) 来增强氨酸乙醇胺 (PE) 定位异构体的检测和成像.
- 将这种改进的技术应用于对复杂组织样本中脂质分布的新生物学见解.
主要方法:
- 组织部分的线下组织臭氧化.
- 通过激光后电离 (MALDI-2) 进行矩阵辅助激光脱吸/电离,以增强信号检测.
- MALDI-2 MSI在小鼠胚胎全身切片和人类结直肠癌活检上的应用.
主要成果:
- MALDI-2有效地增强了PE臭氧化物信号,而没有显著的脆弱臭氧化物的来源碎片化.
- 在小鼠胚胎和人类结直肠癌组织中成功地绘制了定位PE异构体 (例如PE 34:1和PE 36:1).
- 确定了PE异构体在胚胎器官的明显空间分布,在棕色脂肪组织中积累.
- 在结直肠瘤组织中观察到PE 36:1 (Δ9) 异构体的特定丰富.
结论:
- 离线臭氧化和MALDI-2的结合使低丰度脂质的异构成像成为可能,克服了以前的局限性.
- 这种技术对于理解脂质db异构体在正常和癌症代谢过程中的生物学作用具有显著的潜力.
- 该方法很容易被采用,因为它不需要对MALDI质谱仪进行仪器修改.
相关概念视频
MALDI-TOF Mass Spectrometry
6.4K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
6.4K
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)
1.6K
When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
1.6K
Peptide Identification Using Tandem Mass Spectrometry
8.1K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
8.1K
Tandem Mass Spectrometry
2.3K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
2.3K


