分子设计的β-环氧德克斯-酸水凝用于从食品样本中分散固相微提取生物原氨酸
Melody Houshmand1, Saeed Nojavan2
1Department of Analytical Chemistry and Pollutants, Shahid Beheshti University, Evin, Tehran, Iran.
Analytical and bioanalytical chemistry
|December 2, 2025
概括
一种新的绿色方法使用分子建模和分散型固相微提取来检测食品中的生物氨基. 这种可持续的方法增强了食品安全和品质控制的痕迹分析.
科学领域:
- 分析化学 分析化学
- 食品科学 食品科学 食品科学
- 绿色化学 绿色化学
背景情况:
- 准确量化生物胺 (BAs) 对食品质量和安全至关重要.
- 现有的方法可能缺乏敏感性或可持续性.
- 需要新的方法来有效地在复杂的矩阵中确定BA,例如.
研究的目的:
- 开发一种新的,可持续的,高度敏感的方法来确定生物源性氨基.
- 将分子建模与吸附剂选择的实验技术相结合.
- 应用在样本中分析特定BA的方法.
主要方法:
- 分子对接模拟以选基于循环德的吸附剂.
- 使用多变量方法开发和优化分散型固体相微提取 (d-SPME) 程序.
- 在中应用优化的d-SPME方法进行BA分析.
主要成果:
- 鉴定了与酸 (p-β-CDCA) 交叉连接的β-环氧素作为有效的吸附剂.
- 优化的d-SPME条件产生了高的丰富系数 (368-380).
- 该方法表现出色:回收率 (91.6-95.8%),低LOQ (0.45-0.62 ng mL-1),以及良好的精度 (7.3-9.5%).
结论:
- 结合in silico和d-SPME的方法提供了一个绿色,成本效益和敏感的分析工具.
- 开发的方法适用于食品中生物性胺的超微量分析.
- 该战略促进了食品质量评估和安全监测.
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