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CCDC41通过促进Rab11a/Rab7-阳性囊泡与目标膜的融合来驱动卵细胞质进展

Ying Tian^1,2, Jiatong Li³, Jingyi Kang¹

¹Department of Histology and Embryology, School of Basic Medical Sciences, Capital Medical University, Beijing, 100069, P. R. China.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

|December 2, 2025

概括

卷状卷状域含有蛋白41 (CCDC41) 对于卵细胞半细胞分裂至关重要,它调节细胞循环的进展,螺旋转移和染色体分离. 它的耗尽会通过影响囊泡贩运和蛋白酶活性来损害这些过程.

科学领域:

细胞生物学细胞生物学
发展生物学发展生物学
生殖生物学生殖生物学

背景情况:

卷状卷状域含蛋白41 (CCDC41) 是一个心状元件,在卵细胞半分裂中起着未知的作用.
中心和远端附属体对于细胞分裂和乳毛形成至关重要.

研究的目的:

为了阐明CCDC41在卵细胞半分裂中的功能.
调查CCDC41在介质复苏,螺旋转移和染色体分离中的作用.

主要方法:

卵细胞特异性CCDC41耗尽使用淘汰技术.
分析介质性进展,细胞循环调节剂 (循环B1,CDK1,APC/C,CDH1,CDC20) 和动力学.
调查CCDC41的局部化和与囊泡贩运蛋白 (Rab11a,Rab7) 和cathepsin的相互作用.

主要成果:

CCDC41的枯竭导致中介性停止,因为它损害了环素B1的积累和CDK1的激活,这与APC/C失调有关.
CCDC41 knockdown 由于 Rab11a 阳性囊泡融合和 F-actin 定有缺陷而破坏了轴心皮层迁移.
CCDC41的损失通过过早地静止螺旋组装检查点并增强CDC20介导的环素B1降解,加速了亚相开始.
CCDC41局部化到溶酶体,其缺失延迟了Rab7阳性晚期内基因组融合,导致了甲素分散.
抑制甲素B在CCDC41缺少的卵细胞中挽救了介质性进展.

结论:

在卵细胞中,CCDC41对于忠实介质的完成至关重要.
CCDC41通过向 lysosomes 输送 cathepsin 来调节 CDC20 的活动.
CCDC41通过Rab11a-依赖的F-actin anchoring确保了正确的线圈迁移.

关键词:

在CCDC41中使用.在CTSB中,CTSB是CTSB.自自是自的过程.lysosome 溶解酶体是如何形成的鼠标卵细胞是什么

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