相关实验视频

Updated: Jan 9, 2026

Development, Expansion, and In vivo Monitoring of Human NK Cells from Human Embryonic Stem Cells hESCs and Induced Pluripotent Stem Cells iPSCs

Development, Expansion, and In vivo Monitoring of Human NK Cells from Human Embryonic Stem Cells hESCs and Induced Pluripotent Stem Cells iPSCs

Published on: April 23, 2013

开发一种新的体外微核试验,使用人类诱导的多能干细胞衍生T淋巴细胞进行试验

Ryota Kobayashi¹, Katsunori Sasaki², Ryoko Matsuyama²

¹Environmental Health Science Laboratory, Sumitomo Chemical Co., Ltd, Osaka, 554-8558, Japan. kobayashir1@sc.sumitomo-chem.co.jp.

Genes and environment : the official journal of the Japanese Environmental Mutagen Society

|December 2, 2025

概括

这项研究引入了一种新的体外基因毒性测试,使用人类诱导多能干细胞 (hiPSC) 衍生T淋巴细胞. 这些细胞为药物开发中的微核 (MN) 测试提供了稳定可靠的替代方案.

科学领域:

生物技术是生物技术.
毒理学毒理学毒理学
干细胞研究干细胞研究

背景情况:

传统的体外基因毒性测试使用具有异常焦质类型的不朽细胞或具有供体变异性的原发性淋巴细胞.
现有的方法在稳定的供应和正常人体细胞的代表性方面面临挑战.

研究的目的:

开发一种新的体外微核 (MN) 试验,使用人类诱导多能干细胞 (hiPSC) 衍生的T淋巴细胞.
克服当前基因毒性测试模型的局限性.

主要方法:

分化人类诱导多能干细胞 (hiPSCs) 变成T淋巴细胞.
评估T淋巴细胞特征,包括生长,型和自发微核的频率.
使用开发的测定方法评估参考物质的基因毒性.

主要成果:

由hiPSC衍生的T淋巴细胞表现出强大的生长,正常的胆核型和一致的自发微核率.
该试验准确地识别了克拉斯托基因,基因和负物质,与现有文献保持一致.
在各种类别的基因毒性测试中表现出可靠的性能.

结论:

由hiPSC衍生的T淋巴细胞为体外基因毒性评估提供了一个有前途的模型.
这种新的方法为体外微核 (MN) 测试提供了潜在的改进.
该研究提出了用于增强基因毒性评估的原则证明数据.

关键词:

基因毒性基因毒性人类淋巴细胞的人类淋巴细胞在体外微核试验中进行微核试验.诱导的多能干细胞干细胞有毒性有毒性

更多相关视频

Generation of Induced Pluripotent Stem Cells from Human Melanoma Tumor-infiltrating Lymphocytes

Generation of Induced Pluripotent Stem Cells from Human Melanoma Tumor-infiltrating Lymphocytes

Published on: November 11, 2016

An Automated Method to Perform The In Vitro Micronucleus Assay using Multispectral Imaging Flow Cytometry

An Automated Method to Perform The In Vitro Micronucleus Assay using Multispectral Imaging Flow Cytometry

Published on: May 13, 2019

相关概念视频

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...