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Updated: Aug 10, 2026

Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay EMSA and DNA-affinity Precipitation Assay DAPA
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查策略,以识别Cas9变异与更高的HDR活性基于喉毒素的查策略

Daisuke Matsumoto1,2,3, Komari Kubota4, Yu Sato5

  • 1School of Pharmaceutical Sciences, Hiroshima University, 1-2-3 Kasumi Minami-Ku, Hiroshima, 734-8553, Japan. matsumoto-ds@igakuken.or.jp.

Journal of biomedical science
|December 3, 2025
PubMed
概括

研究人员开发了一种查系统,以找到改善同质导向修复 (HDR) 的Cas9突变,以实现精确的基因编辑. 确定了一种具有增强HDR效率的新型SpCas9突变,从而提升了基因治疗的潜力.

关键词:
双菌毒素的使用方法同质导向维修是同质导向维修的一种方式.查检查 查检查 查检查

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相关概念视频

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...