环境污染物对抗微生物耐药性基因转移的影响:比较分析
Maomao Xie1, Jie Jiang1, Zhiqiang Xiong1
1State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Suzhou Medical College, Soochow University, Suzhou 215123, China.
Environmental science & technology
|December 3, 2025
概括
一个新的高通量选平台有效地评估了环境污染物如何影响抗菌素耐药性基因 (ARG) 转移. 只有某些抗生素显著增强了ARG转移,揭示了可以为监管干预提供信息的机制.
科学领域:
- 环境科学 环境科学
- 微生物学 微生物学
- 分子生物学分子生物学
背景情况:
- 横向基因转移 (HGT) 是生态系统中抗菌素耐药性基因 (ARG) 传播的主要机制.
- 了解环境污染物如何影响ARG转移对于评估生态风险至关重要,但目前的方法缺乏高通量能力.
研究的目的:
- 开发和验证一个高通量选 (HTS) 平台,系统评估各种环境污染物对ARG转移的影响.
- 识别影响ARG水平基因转移速度的特定污染物.
主要方法:
- 设计了一种 *Escherichia coli* 供体菌株,其中含有携带 ARG 的结合性等离子体和传输响应的光记者系统.
- 利用HTS平台对136种环境污染物进行选,以检测它们对ARG转移到受体细菌的影响.
- 采用机械分析来研究涉及污染物介导的合增强的分子通路.
主要成果:
- 与传统方法相比,HTS平台展示了优越的速度,效率和可重复性.
- 在136种测试的污染物中,只有4种,特别是某些抗生素,在环境相关度下显著增强了ARG转移.
- 机制研究表明,抗生素会诱导不对称的压力,激活捐赠细胞中的IV型分泌系统,促进结合.
结论:
- 开发的HTS平台为评估污染物对ARG传播的影响提供了强大而高效的工具.
- 研究结果强调了某些抗生素在加速ARG转移中的特殊作用,为环境风险提供了洞察力.
- 这项研究有助于更好地了解ARG环境风险,并支持制定有针对性的监管策略.
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