击败LncRNA PART1通过调节miR-638的表达来抑制因缺血性中风引起的神经损伤
Guangfei Xu1, Hewen Li2, Ting Huang3
1Department of Geriatrics, Nantong Hospital of Traditional Chinese Medicine (Nantong Affiliated Hospital, Nanjing University of Traditional Chinese Medicine), Nantong City, China.
概括
击败PART1通过上调miR-638.8来防止缺血性中风 (IS). 这一发现为IS提供了新的治疗策略,这种疾病会导致严重的残疾和死亡.
科学领域:
- 神经科学是一个神经科学.
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 缺血性中风 (IS) 是全球死亡和残疾的主要原因.
- 了解IS背后的分子机制对于开发有效的治疗方法至关重要.
研究的目的:
- 研究PART1和miR-638在IS中的作用.
- 探索针对IS中PART1的治疗潜力.
主要方法:
- 定量实时PCR测量基因表达.
- 生物化学测试 (SOD,MDA,LDH,IL-6,TNF-α) 的ELISA套件. 这是一个非常好的方法.
- 细胞活力测试 (CCK-8),亡的流细胞计,光酶记者和分子相互作用的RIP测试.
主要成果:
- 在IS患者和OGD/R模型中,PART1是上调的,miR-638是下调的.
- PART1 knockdown 增强了细胞活力,减少了细胞亡,并调节了炎症标记物和氧化应激.
- PART1负调节的miR-638和miR-638的抑制减弱了PART1敲击的保护作用.
结论:
- PART1 Knockdown通过调节miR-638.8来缓解IS引起的神经损伤.
- 针对PART1/miR-638轴为IS提供了一个潜在的治疗策略.
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