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两个新型的S-甲基转移酶在Actinomycetota中产生二甲基硫化物

Ruihong Guo^1,2, Zihua Guo^1,3, Yi Zhou¹

¹Frontiers Science Center for Deep Ocean Multispheres and Earth System, and College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

|December 3, 2025

概括

在Actinomycetota细菌中发现了两种新型酶MddM1和MddM2,它们可以从有毒的硫气体中产生二甲基硫化物 (DMS). 这些酶对于硫循环和在不同的环境中耐受压力至关重要.

科学领域:

微生物学与环境科学微生物学与环境科学
生物化学和分子生物学

背景情况:

硫化 (H2S),甲乙醇 (MeSH) 和二甲基硫化 (DMS) 等硫气体在全球硫循环,微生物化学反应和气候调节中发挥着至关重要的作用.
微生物利用MddA和MddH等酶将细胞毒性H2S和MeSH转化为无毒的DMS,主要在海洋和陆地环境中.
在丰富的细菌族群中,特别是Actinomycetota中,DMS生产的酶机制仍然在很大程度上未被探索,可能低估了它们对硫循环的贡献.

研究的目的:

在Actinomycetota.ta.中识别负责H₂S和MeSH S-甲基化的新型酶.
研究这些酶在*Mycolicibacterium poriferae*中排毒,氧化应激减轻和DMS产生中的作用.
评估这些新发现的酶在各种细菌群和环境中的流行程度和生态意义.

主要方法:

生物信息分析和酶试验用于识别和表征新型S-腺氨酸-氨酸依赖甲基转移酶 (MddM1和MddM2) 在* Mycolicibacterium poriferae* ZYF656.6.
基因表达分析 (转录研究) 在不同的条件下 (H₂S,MeSH,氧化应激) 和在大肠杆菌中的异质表达,以评估酶功能和应激耐受性.
转基因和16S rRNA基因测序分析,以确定mddM1和mddM2基因在不同细菌群和环境样本 (土壤,沼泽沉积物) 的分布.

主要成果:

在*Mycolicibacterium poriferae*中发现和描述了两个新的H₂S和MeSH S-甲基转移酶,MddM1和MddM2.
证据表明,MddM1和MddM2可以排毒H₂S和MeSH,减轻氧化应激,正如应激下诱导的转录和表达这些基因的大肠杆菌增强的耐受性所表明的那样.
MddM1和/或MddM2酶存在于超过50%的Actinomycetota中,包括*Streptomyces*物种,还存在于Chloroflexota,Acidobacteriota和Proteobacteria中,mddM1在土壤和沼泽沉积物中更为丰富和普遍.

结论:

动菌株含有新型酶 (MddM1,MddM2),它们从H₂S和MeSH显著促进DMS的产生,在硫循环中发挥关键作用.
这些酶增强了细菌对有毒硫化合物和氧化应激的耐受性,突出了它们在微生物生存和适应中的重要性.
这些酶的广泛分布,特别是MddM1在Actinomycetota中,强调了该类对全球DMS生产和硫生物化学的重大,以前被低估的贡献.

关键词:

这就是Actinomycetota.通过S-甲基转移酶.甲基二硫化二甲基硫化物甲乙醇是一种甲乙醇.硫化物硫化物是什么

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