在动脉样硬化和血管损伤中, Gli1+ 进阶干细胞对光滑肌肉细胞的贡献

相关实验视频

Updated: Jan 9, 2026

Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements In Vitro

Published on: February 15, 2022

4.2K

Haixiao Wang^1,2,3, Xiuzhen Huang⁴, Jialing Mou⁴

¹Pediatric Research Institute, Pediatric Discipline Group, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, 325000, China.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

|December 3, 2025

概括

在动脉样硬化中,突发性干细胞 (ASC) 不会形成光滑肌细胞 (SMC). 然而,ASC确实在血管损伤后产生SMC,表明治疗向的上下文依赖角色.

科学领域:

血管生物学血管生物学
干细胞生物学干细胞生物学
动脉样硬化研究动脉样硬化研究

背景情况:

由Gli1表达标记的突发性干细胞 (ASC) 假设有助于动脉样硬化中的血管光滑肌肉细胞 (SMC) 种群.
在动脉样硬化期间,Gli1+ ASCs对SMCs的确切贡献和血统仍在争论中.

研究的目的:

为了澄清 Gli1+ ASCs 在动脉样硬化病变中的 SMCs 发展中的精确作用.
在血管损伤模型中研究 Gli1+ ASC 的上下文依赖差异化潜力.

主要方法:

使用Gli1-CreER血统追踪工具在动脉样硬化模型中追踪ASC.
开发了一种双重复合酶介导的遗传策略,专门标记Gli1+ ASC,并排除SMC的异胎标记.
在动脉样硬化和血管解剖损伤模型中进行了命运映射研究.

主要成果:

最初的谱系追踪表明,Gli1+细胞对动脉样硬化中的SMCs的贡献很小.
双系追踪最终表明,Gli1+ ASCs不会在动脉样硬化斑块中的SMCs中发挥作用.
发现Gli1+ ASCs在血管解剖损伤后分化为显著的SMC群体.

结论:

Gli1+ ASCs不会在动脉样硬化斑块中的SMC群体中发挥作用,这挑战了以前的假设.
Gli1+ ASCs在SMC中的差异化潜力取决于上下文,在血管损伤后是显著的,但不是动脉样硬化.
这些发现需要重新评估动脉样硬化治疗干预的细胞点.

关键词:

动脉样硬化动脉样硬化宿命绘制 - 绘制命运的地图谱系追踪谱系追踪谱系追踪干细胞是一种干细胞.血管光滑肌肉细胞细胞

相关概念视频

01:30

Atherosclerosis I: Introduction

818

Atherosclerosis is a progressive disorder characterized by the buildup of plaques on the arterial inner wall, causing them to narrow and harden over time. These plaques comprise lipids, calcium, blood components, carbohydrates, and fibrous tissue. The process primarily affects the intima of large and medium-sized arteries, reducing blood flow in any artery.Etiology and risk factorsThe cause of atherosclerosis is multifactorial, involving a complex interplay among endothelial injury, lipid...

818

01:24

Regulation of Angiogenesis and Blood Supply

3.3K

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...

3.3K

01:19

Mesenchymal Stem Cells

5.5K

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...