Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA-seq03:21

RNA-seq

11.7K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
11.7K
Ribosome Profiling02:24

Ribosome Profiling

4.0K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Decreased degree of adipocyte differentiation in visceral adipose tissue contributes to metabolic dysfunction-associated steatotic liver disease.

Nature communications·2026
Same author

Decoding sequence determinants of gene expression in diverse cellular and disease states.

Nature methods·2026
Same author

The Rayleigh Quotient and Contrastive Principal Component Analysis II.

bioRxiv : the preprint server for biology·2026
Same author

Hybrid crosses reveal a cell-type-specific landscape of mouse regulatory variation.

bioRxiv : the preprint server for biology·2026
Same author

Lessons from single cell omics: admixed American ancestry and sex confer cardiometabolic disease risk in Mexicans.

Genome medicine·2026
Same author

Disruption of Polycystin Ciliary Localization and Channel Function by Autosomal Dominant Polycystic Kidney Disease-Causing Polycystin-1 Variants.

Journal of the American Society of Nephrology : JASN·2026
Same journal

Layered social competition coordinates reproductive hierarchy formation in ants.

bioRxiv : the preprint server for biology·2026
Same journal

Combination epigenetic-targeted therapy increases the immunogenicity of poorly immunogenic sarcomas.

bioRxiv : the preprint server for biology·2026
Same journal

Loss of LanC-like proteins delays post-injury regeneration of aging skeletal muscles.

bioRxiv : the preprint server for biology·2026
Same journal

Integrative Transfer Network: Deep Transfer Learning Across Populations and Prediction Targets.

bioRxiv : the preprint server for biology·2026
Same journal

Confidence-supported label-free metabolic imaging with FPhaS phase autofluorescence microscopy.

bioRxiv : the preprint server for biology·2026
Same journal

Sequence-encoded autoinhibition couples mRNA decapping activity to phase separation.

bioRxiv : the preprint server for biology·2026
查看所有相关文章

相关实验视频

Updated: Jan 9, 2026

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

7.0K

从多变体单细胞RNA测序数据中确定基因特异性.

Nikhila P Swarna1, A Sina Booeshaghi2, Elisabeth Rebboah3,4

  • 1Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.

bioRxiv : the preprint server for biology
|December 3, 2025
PubMed
概括
此摘要是机器生成的。

我们开发了 ember,这是一种用于识别单细胞基因组学中的基因特异性的新方法. 恩伯揭示了老鼠组织和人类细胞中复杂的基因表达模式,突出了取决于环境的基因功能.

更多相关视频

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
10:12

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

19.0K
Low-input Nucleus Isolation and Multiplexing with Barcoded Antibodies of Mouse Sympathetic Ganglia for Single-nucleus RNA Sequencing
10:44

Low-input Nucleus Isolation and Multiplexing with Barcoded Antibodies of Mouse Sympathetic Ganglia for Single-nucleus RNA Sequencing

Published on: March 23, 2022

4.8K

相关实验视频

Last Updated: Jan 9, 2026

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

7.0K
Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
10:12

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

19.0K
Low-input Nucleus Isolation and Multiplexing with Barcoded Antibodies of Mouse Sympathetic Ganglia for Single-nucleus RNA Sequencing
10:44

Low-input Nucleus Isolation and Multiplexing with Barcoded Antibodies of Mouse Sympathetic Ganglia for Single-nucleus RNA Sequencing

Published on: March 23, 2022

4.8K

科学领域:

  • 基因组学就是基因组学.
  • 计算生物学 计算生物学
  • 生物信息学是一种生物信息学.

背景情况:

  • 在单细胞基因组学中,确定特定于生物类别或实验条件的基因至关重要.
  • 现有的基因特异性分析方法缺乏标准化的公理方法.

研究的目的:

  • 为测量基因特异性开发一种强大且公理化的方法.
  • 将这种方法应用于在小鼠和人类数据集中发现新的基因表达模式.

主要方法:

  • 开发了 ember (Entropy Metrics for Biological ExploRation),一种用于基因特异性测量的公理方法.
  • 从八个小鼠组织和创始菌株的单细胞RNA测序数据中应用了 ember.
  • 利用无监督学习的度指标来识别专门的基因.
  • 对人类外周血液单核细胞 (PBMC) 数据集进行了扩展的 ember 分析.

主要成果:

  • 恩伯是唯一一种满足基因特异性的四个关键性质的方法.
  • 鼠标基因特异性往往是不直观的,有上下文依赖的家政基因和菌株驱动的细胞类型切换.
  • 发现了男性生殖腺和脏的共同基因,以及特定阶段的脏基因.
  • 人类PBMC变异集中在古典单细胞中,已确定性别,年龄和祖先特定的基因.

结论:

  • 恩伯为单细胞基因组学中的基因特异性分析提供了一个强大的,公理性的工具.
  • 这项研究揭示了老鼠和人类复杂和上下文依赖的基因表达模式.
  • 这些发现提供了对遗传变异的洞察力,并为未来使用小鼠模型的研究提供了路线图.